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Showing: 211 - 240 of 309 results

  13. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid
    #162950
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    162950-plasmid-map-sequence-id-331914

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  17. Gene Trap - 24XMS2 Lentiviral Vector

    Plasmid
    #174198
    Purpose
    Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.
    Depositor
    Daniel Larson
    Article
    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.
    Insert
    iRFP
    Use
    Lentiviral
    Tags
    24X MS2 hairpins, Blasticidin, and T2A
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    174198-plasmid-map-sequence-id-371140

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  18. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  19. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  20. Gene Trap - 24XPP7 Lentiviral Vector

    Plasmid
    #174199
    Purpose
    Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.
    Depositor
    Daniel Larson
    Article
    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.
    Insert
    iRFP
    Use
    Lentiviral; Vector
    Tags
    24X PP7 hairpins, Blasticidin, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    174199-plasmid-map-sequence-id-343727

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  22. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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  23. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

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  27. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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Showing: 211 - 240 of 309 results