We narrowed to 777 results for: PI
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Plasmid#86596PurposeExpresses mCherry-tagged PH(WT) domain of human Akt2DepositorAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSH124-ssr
Plasmid#198025PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Ap/Cb resistance cassette
ExpressionBacterialPromoterApR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
ExpressionBacterialPromoterlacIq-PtrcAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-hlPYK WT
Plasmid#187975PurposeExpresses the human liver pyruvate kinaseDepositorInserthlPYK WT
TagsHexahistagExpressionBacterialPromoterT7Available SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
BFP-Dcp1a
Plasmid#153973PurposeFluorescent protein fused to mRNA decapping enzyme 1aDepositorAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT9651-BEAR-GFP
Plasmid#162989PurposeBEAR target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt1(WT)
Plasmid#86631PurposeExpresses mCherry-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT9752-BEAR-mScarlet
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEditA
Plasmid#207531PurposePlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpRDepositorInsertPlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB-Dcp1b
Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-PIP5K1C_Kina-HD
Plasmid#202741Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-PIP5K1C_Kina-HDD316K
Plasmid#202742Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)
ExpressionMammalianAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH(Btk)
Plasmid#86635PurposeExpresses eGFP-taggd PH domain of BtkDepositorInsertBtk PH domain (BTK Human)
ExpressionMammalianAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832AAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 GFP-PIK3C2B
Plasmid#162001PurposeLentiviral expression vector containing PIK3C2B tagged with GFPDepositorInsertPIK3C2B (PIK3C2B Human)
UseLentiviralTagseGFPMutationsiRNA resistance to siRNA#2 with 4 silent mutatio…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
Promoterp14GAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAT9650-BEAR-GFP-preedited
Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS44i8GM
Plasmid#207527PurposeConditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
UseCRISPR; Pseudomonas vectorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationExpressionBacterialPromoterPmAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSK418
Plasmid#205131PurposeExpression of GFP-2xFYVE in DictyosteliumDepositorAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 GFP-PIK3C2A
Plasmid#162000PurposeLentiviral expression vector containing PIK3C2A tagged with GFPDepositorAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT15415-BEAR-GFP-target-mCherry
Plasmid#162995Purposeplasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the BEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFKBPmCherry-Akt2(K14A)
Plasmid#86597PurposeExpresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutantDepositorInsertFKBB-mCherry-Akt2(K14A) (AKT2 )
ExpressionMammalianAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)
Plasmid#86595PurposeExpresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutantDepositorInsertAkt2(K14A/R25E) (AKT2 )
ExpressionMammalianAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB128
Plasmid#205137PurposeExpression of mCherry-2xFYVE in DictyosteliumDepositorAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT9751-BEAR-mCherry-preedited
Plasmid#162993PurposeBEAR control plasmid with split mCherry and intact 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(T309A)
Plasmid#86625PurposeExpresses eGFP-tagged human Akt2(T309A) phosphomutantDepositorInsertAkt2(T309A) (AKT2 Human)
ExpressionMammalianAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorInsertAkt1(D323A/D325A) (AKT1 Human)
ExpressionMammalianAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PH1(K14A)
Plasmid#86600PurposeExpresses mCherry-tagged PH(K14A) domain of human Akt1DepositorInsertK14A mutant of Akt1 PH domain (AKT1 Human)
ExpressionMammalianAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT15416-BEAR-mScarlet-target-BFP
Plasmid#162996Purposeplasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP markerDepositorInsertsgRNA targeting the BEAR-mScarlet plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT9753-BEAR-mScarlet-preedited
Plasmid#162994PurposeBEAR control plasmid with split mScarlet and intact 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only