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Plasmid#15173DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only
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P66m
Plasmid#120954PurposeMoClo golden gate assembly AB part for pRpa-AM (Adam Meyer's improved pRPA promoter from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertpRpa Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P37m
Plasmid#120947PurposeMoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpRpa* from Scott and Hasty 2016
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C70m
Plasmid#120982PurposeMoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).DepositorInsertRpaR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C61m
Plasmid#120977PurposeMoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRpaR from Scott and Hasty 2016
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
G-RCAS (A)
Plasmid#15182DepositorInsertGateway cassette
UseRNAi and Retroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMM95
Plasmid#176615PurposeExtracellular domain truncated. Used to test SIRPA exclusion from the phagocytic synpase.DepositorInsertFRBext-GFPint
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMM98
Plasmid#176617PurposeContains 3 fibcon repeats to observe SIRPA exclusion from the phagocytic synapseDepositorInsertFRB-FIB3ext-GFPint
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H mCherry-R5 AmpR
Plasmid#225141PurposeRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 160N, mCherry
UseTagsAg43 protein fused to silaffin-R5 and mCherryExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_OHu19986C_myc tag
Plasmid#183174Purposeused to make AAV vectors for hsTRPA1 tagged with mycDepositorInserthsTRPA1 (TRPA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1345
Plasmid#142788PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRPA1 (RPA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H sfGFP-R5 AmpR
Plasmid#225139PurposeRecombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 protein fused to silaffin-R5 and sfGFP
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF1348
Plasmid#141814PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRPA2 (RPA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3227
Plasmid#144703PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRPA2 (RPA2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1TP9
Plasmid#234200PurposeHeterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coliDepositorInsert1TP9 (LOC7476296 Populus trichocarpa)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET9d-hnRNP-A1
Plasmid#23026DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCC1R
Plasmid#213497PurposeEncodes the receptor array and inducible aiiA gene of the three-node Turing circuitDepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_4IPC
Plasmid#234229PurposeHeterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coliDepositorInsert4IPC (RPA1 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmVen-IRES
Plasmid#211428PurposeVector backbone for coupled mammalian expression of N-terminal Venus fusion protein and another proteinDepositorInsertIRES sequence (RPA2 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only