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7749 Bicistronic_ires_hygro

Plasmid

#64374

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL.gp91.EF1aIntron.eGFP

Plasmid

#30466

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

CMVenh/gp91/EF1aIntron/eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAcBac1-3nitroY-A7-RS

Plasmid

#141173

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

Insert

A7 3nY tRNA synthetase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P186T L274R N311G C313N

Promoter

CMV

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE_shLARP7

Plasmid

#113538

Purpose

Retroviral vector designed to knock down MePCE and LARP7 simultaneously.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE and shLARP7 (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron-G

Plasmid

#36887

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron-T

Plasmid

#36885

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-267

Plasmid

#68770

Purpose

Sensor pair 1 V1

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-terminal Sensor 1 V1
N-Terminal Sensor 1 V1

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pRRL.gp91.Syn.Intron.eGFP

Plasmid

#30467

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

gp91/Syn.Intron/eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro BRIT1

Plasmid

#16206

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

shBRIT1 (MCPH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pRS416-ACT1_intron

Plasmid

#127602

Purpose

low-copy URA-selectable plasmid, ACT1 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ACT1 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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8200 Bicistronic_GFP_ires_hygro

Plasmid

#64375

Purpose

This a retroviral expression plasmid expressing GFP along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

EGFP

Use

Retroviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-271

Plasmid

#68772

Purpose

Sensor pair 1 V3

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V3
N-Terminal Sensor 1 V3

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pOD2049-csnControl

Plasmid

#100007

Purpose

MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Inserts

zif-1 (zif-1 Nematode)
unc-119

Use

Targeting vector for mos1 transposon mediated sin…

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-148

Plasmid

#68749

Purpose

ZF8/ZF7-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF8/ZF7-VP64
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-149

Plasmid

#68750

Purpose

ZF6/ZF10-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF6/ZF10-VP64
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL.CMVenh.gp91.EF1aIntron.eGFP

Plasmid

#30469

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

CMVenh/gp91.EF1alntron.eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-127

Plasmid

#68733

Purpose

ZF5-TF with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF5-VP64
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge