We narrowed to 1,219 results for: cas12

Showing: 141 - 160 of 1219 results

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pYPQ134-RZ-Cas12j2

Plasmid

#173930

Purpose

Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L2-dMb2Cas12a

Plasmid

#177684

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a_crRNA Td2

Plasmid

#176255

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR2

Plasmid

#176248

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR1

Plasmid

#176247

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR3

Plasmid

#176249

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ288 (BoCas12a)

Plasmid

#138125

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BoCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ283 (MlCas12a)

Plasmid

#138115

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MlCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ285 (Lb5Cas12a)

Plasmid

#138120

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ286 (CMaCas12a)

Plasmid

#138122

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

CMaCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ287 (BsCas12a)

Plasmid

#138123

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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BPK2013-BsCas12b

Plasmid

#121952

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-UltraCas12a-6xNLS

Plasmid

#209023

Purpose

Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

UltraCas12a-6xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

M537R/F870L

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSIBR2.0-818-Cas12a-NT

Plasmid

#235168

Purpose

Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

Depositor

Constantinos Patinios

Article

Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

Insert

SIBR2.0-818-Cas12a

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-FnCas12a-TEV

Plasmid

#174658

Purpose

FnCas12a expression plasmid

Depositor

Jin Wang, Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

FnCas12a

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas12a

Plasmid

#124891

Purpose

obtaining full Cas12a RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas12a

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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