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pDM358_DPAKa(GBD)-YFP

Plasmid

#128700

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM326_DPAKa(GBD)-YFP

Plasmid

#128701

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM304_DPAKa(GBD)-YFP

Plasmid

#128699

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_GAP(R527L_K566A)

Plasmid

#222617

Purpose

Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Point mutations R527L and K566A (Rac1-specific GA…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-pGBD

Plasmid

#26735

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Rac (rac1 Frog)

Use

Reporter for rac

Tags

GFP

Expression

Mammalian

Mutation

Rac binding domain of rhotekin

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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CFP-PBD

Plasmid

#11394

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
YFP-PBD

Plasmid

#11407

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 PBD (PAK1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
JB42

Plasmid

#128924

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rac1QL-BirA*-HA-MAO (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB54

Plasmid

#128925

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rac1TN-BirA*-HA-MAO (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Rac2

Plasmid

#11393

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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mCherry-SEpHluorin

Plasmid

#32001

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

mCherry

Use

Tags

Superecliptic pHluorin

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-Cdc42

Plasmid

#11392

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

cell division cycle 42 (CDC42 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Rac2(V12)

Plasmid

#11397

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glycine 12 changed to Valine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(N17)

Plasmid

#11398

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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CFP-PBD(H83,86L)

Plasmid

#11402

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Obtained with Histidine residues 83 and 86 change…

Promoter

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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