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lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Brevican [N294A/10R-2b]

Plasmid

#220429

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N294A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody. (Bcan Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Reverse-Donor-BsaI

Plasmid

#197843

Purpose

Donor vector to restore L2 destination via BsaI (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Reverse-Donor-Esp3I

Plasmid

#197844

Purpose

Donor vector to restore L2 destination via Esp3I (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L1-Reverse-Donor-Esp3I

Plasmid

#197845

Purpose

Donor vector to restore L1 destination via Esp3I (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HIV-Rev-M10_O

Plasmid

#147112

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev_O

Plasmid

#147113

Purpose

Mammalian Expression of HIV-Rev

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mNG

Plasmid

#189722

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-TMED3-Reverse-Complement

Plasmid

#136018

Purpose

The Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

TMED3 3'UTR (Reverse Complement) (TMED3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-ZC3H15-Reverse-Complement

Plasmid

#136019

Purpose

The Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

ZC3H15 3'UTR (Reverse Complement) (ZC3H15 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx-rev-post-mGRASP-2A-dTomato

Plasmid

#34913

Purpose

JxON-post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2-tdtomato

Plasmid

#18917

Purpose

Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-tdtomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdtomato

Expression

Mutation

Promoter

CAG

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-oChIEF-tdTomato

Plasmid

#30541

Purpose

Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

Depositor

Rory McQuiston

Article

Bell et al Neuropharmacology. 2011 Aug 25.

Insert

oChIEF-tdtomato

Use

AAV and Cre/Lox

Tags

tdTomato

Expression

Mutation

Promoter

CAG

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AA0250-mCherry-2a-hM4D-nrxn1a rev

Plasmid

#60544

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Inserts

mCherry
muscarinic receptor 4, axon targeted variant (CHRM4 Human)

Use

AAV and Cre/Lox

Tags

2xHA

Expression

Mutation

Promoter

CAG

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-Rev-3xGFP

Plasmid

#122500

Purpose

Cre dependent GFP expression.

Depositor

Kimberly Ritola

Insert

3x GFP

Use

AAV

Tags

Expression

Mutation

each GFP has unique nt sequence

Promoter

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta rev

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Use

Tags

Expression

Mutation

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pattB-synaptobrevin-7m1-QFBDADM1-hsp70

Plasmid

#46116

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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