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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 613 results for: ski

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Showing: 141 - 160 of 613 results

  6. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  9. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  10. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  12. p404TDH3

    Plasmid
    #15973
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15973-plasmid-map-sequence-id-8458

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  16. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid
    #52495
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52495-plasmid-map-sequence-id-81430

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  19. p404TEF1

    Plasmid
    #15972
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15972-plasmid-map-sequence-id-8457

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Showing: 141 - 160 of 613 results