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pLVX-CMV-Galpha-i1 ONE-GO

Plasmid

#189735

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i1 ONE-GO
GNAI1 (Gnai1 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCL-Eco

Plasmid

#12371

Depositor

Inder Verma

Article

Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

Insert

gag/pol/env

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

TRE3G-Bi-directional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-M2rtTA

Plasmid

#20342

Purpose

Lentiviral plasmid expressing the reverse tetracycline transactivator

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

M2rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-CD19 puro

Plasmid

#196634

Purpose

Express CD19

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

CD19 (CD19 Human)

Use

Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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