We narrowed to 12,292 results for: BASE

1. qTAG-C-ultraID-Blast

   Plasmid

   #207715

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDN-Cherry-sgRNA

   Plasmid

   #221387

   Purpose

   episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   none, cloning vector for introduction of targeting sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pS2513·PHP

   Plasmid

   #122590

   Purpose

   Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

   Depositor

   Pablo Ivan Nikel

   Article

   Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

   Insert

   phIorine2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pem7

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1α-Cre-2A-mTagBFP2

   Plasmid

   #207746

   Purpose

   Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-PGK-mTagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-C-sTagRFP-Puro

   Plasmid

   #207723

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCS4333 yeTadA1.0-T7 RNAP

    Plasmid

    #137735

    Purpose

    pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

    Depositor

    Christina Smolke

    Article

    Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

    Insert

    yeTadA1.0-T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    E366G (See depositor comment below)

    Promoter

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF-AncBE4max

    Plasmid

    #138270

    Purpose

    Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His-Tev-UbE2D3

    Plasmid

    #186805

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE2D3 (UBE2D3 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-KO-Blast

    Plasmid

    #227263

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCN34e-ktrA-yfp

    Plasmid

    #227640

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pMK462 (Hygro-P2A-mAB)

    Plasmid

    #214388

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-C-mScarlet-Blast

    Plasmid

    #207711

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

    Plasmid

    #209293

    Purpose

    mitoBE expression vector, target mtND4 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MscL(G22S)-c-term-cpGFP-ERexp

    Plasmid

    #195346

    Purpose

    Control plasmid for the MscL tension biosensor

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)-c-term-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd2

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

    Plasmid

    #209294

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    SOD2-3xHA-TALE(RNR2-Left)-MutH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pST5552

    Plasmid

    #36255

    Purpose

    Mycobacteria replicating vector expressing eGFP.

    Depositor

    Jessica Seeliger, Carolyn Bertozzi

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Insert

    eGFP
    

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ModifiedHsp60

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC-DEST-hPROX1-tdTomato-PuroTK

    Plasmid

    #141027

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-4mtD3cpv

    Plasmid

    #36324

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
    

    Use

    Tags

    First 29 aa of human COX subunit 8a

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG FUCCI

    Plasmid

    #175266

    Purpose

    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

    Depositor

    James Monaghan

    Article

    Duerr et al Development. 2022 Mar 15;149(6). pii: 27

    Insert

    FUCCI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPRIME-CMV-Neo-recipient

    Plasmid

    #11659

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling Neomycin expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Neo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. qTAG-N-Blast-miniTurbo

    Plasmid

    #207681

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pSP6-EBNA(2A+DBD)

    Plasmid

    #98749

    Purpose

    In vitro transcription of modified EBNA1 mRNA

    Depositor

    Jim Vadolas, Sara Howden

    Article

    Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

    Insert

    Modified EBNA1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FRB_SCF2_LD6_CTEV

    Plasmid

    #58879

    Purpose

    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FKBP_SCF2_LD6_NTEV

    Plasmid

    #58878

    Purpose

    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSGAb-spe

    Plasmid

    #122000

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid

    #170578

    Purpose

    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDKL5 (CDKL5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    E854G- please see depositor comments

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BmTyr-Flag

    Plasmid

    #220779

    Purpose

    non-targeted BmTyr throughout the cytoplasm

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    Bacillus megaterium Tyrosinase
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHR-hSyn-EGFP

    Plasmid

    #114215

    Purpose

    Lentiviral vector (control) that targets expression of EGFP to neurons

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge