We narrowed to 4,045 results for: golden gate

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pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227907

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227923

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227908

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227909

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227910

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227916

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227891

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

VPS35 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227892

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

EEA1 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227900

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227901

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227902

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227904

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227905

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec

Plasmid

#239600

Purpose

expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec

Plasmid

#239599

Purpose

expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec

Plasmid

#239598

Purpose

expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec

Plasmid

#239597

Purpose

expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

Plasmid

#239596

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPlxnb2-2 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239589

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPlxnb2-3 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239590

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239588

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

Plasmid

#216166

Purpose

Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)

Use

Lentiviral

Tags

EGFP

Promoter

phU6, ph7SK, phH1, pmU6

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227925

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227926

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227914

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227918

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227920

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227921

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227894

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC61B homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227896

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227897

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

NCLN homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227903

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p2R3z-BASI-ccdB-BSAI

Plasmid

#118389

Purpose

Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

ccdB

Use

Unspecified

Mutation

See Depositor Comments Section

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB.PRC2.AEBP2

Plasmid

#234580

Purpose

Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3]

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4, AEBP2 [Iso 3] (EZH2, SUZ12, RBBP4, EED, AEBP2 Human)

Expression

Insect

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGGD_RT_li_Venus

Plasmid

#136973

Purpose

Goldengate/Greengate C-tag module, containing Venus fused to a flexible linker

Depositor

Klaus Theres

Article

Lopez et al Plant Cell. 2021 Aug 13;33(7):2412-2430.

Insert

Venus

Expression

Bacterial

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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