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Plasmid#227647PurposepTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_AT
Plasmid#196243PurposeBackbone plasmid for cloning sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBB328
Plasmid#221533PurposePlasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.DepositorInsertsvioABCE
Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
Tetracycline antibiotic cassette from pRK415
UseTagsExpressionBacterialMutationPromoterNone, contains RBS siteAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRK1
Plasmid#173730Purposedonor plasmid for SapTrap assemblyDepositorInsertrps-0 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK143
Plasmid#173737Purposedonor plasmid for SapTrap assemblyDepositorInsertelt-2 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK151
Plasmid#173739Purposedonor plasmid for SapTrap assemblyDepositorInsertrps-0 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK142
Plasmid#173736Purposedonor plasmid for SapTrap assemblyDepositorInserthsp-16.48 promoter
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK150
Plasmid#173738Purposedonor plasmid for SapTrap assemblyDepositorInsertunc-54 3' UTR
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK153
Plasmid#173740Purposedonor plasmid for SapTrap assemblyDepositorInsertunc-54 3' UTR
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1411
Plasmid#167638PurposeExpress FepA I14C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA I14C
UseTagsExpressionBacterialMutationfepA with I14C mutation (Mutation is I36C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1383
Plasmid#167639PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V15C
UseTagsExpressionBacterialMutationfepA with V15C mutation (Mutation is V37C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1384
Plasmid#167641PurposeExpress FepA V16C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V16C
UseTagsExpressionBacterialMutationfepA with V16C mutation (Mutation is V38C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1416
Plasmid#167643PurposeExpress FepA T17C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T17C
UseTagsExpressionBacterialMutationfepA with T17C mutation (Mutation is T39C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1583
Plasmid#167645PurposeExpress FepA D34C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C
UseTagsExpressionBacterialMutationfepA with D34C mutation (Mutation is D56C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1382
Plasmid#167647PurposeExpress FepA G54C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C
UseTagsExpressionBacterialMutationfepA with G54C mutation (Mutation is G76C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP718
Plasmid#167636PurposeExpress FepA T13C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T13C
UseTagsExpressionBacterialMutationfepA with T13C mutation (Mutation is T35C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1410
Plasmid#167634PurposeExpress FepA D12C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D12C
UseTagsExpressionBacterialMutationFepA with D12C mutation. (Numbering based on star…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ2039_N2only
Plasmid#140682PurposeControl (“C”) lacking sg-3DepositorInsertControl (“C”) lacking sg-3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2040_N2only
Plasmid#140683PurposeControl (“C”) lacking sg-4DepositorInsertControl (“C”) lacking sg-4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only