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Showing: 1581 - 1600 of 32215 results

  1. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  4. pLenti GW V5 Eco/Dam hum LaminA

    Plasmid
    #182673
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.
    Depositor
    Bas van Steensel
    Article
    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:
    Insert
    Lamin A (LMNA Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Mutation
    Promoter
    HSP
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182673-plasmid-map-sequence-id-357405

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  8. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid
    #141198
    Purpose
    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines
    Depositor
    Simon Bekker-Jensen
    Article
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Insert
    MAP3K20 (MAP3K20 Human)
    Use
    Tags
    Strep, HA
    Expression
    Mammalian
    Mutation
    deletion of last 27 amino acids and amino acids 6…
    Promoter
    CMV
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141198-plasmid-map-sequence-id-300399

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  9. cmamxA2-GFP

    Plasmid
    #107197
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted
    Depositor
    Volker Gerke, Ursula Rescher
    Article
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Insert
    cmannexin A2 (ANXA2 Human)
    Use
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    all type II Ca2+ binding sites deleted ( D161A, E…
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107197-plasmid-map-sequence-id-209669

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  10. p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

    Plasmid
    #52216
    Purpose
    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation
    Depositor
    Eric Huang
    Article
    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2
    Insert
    HIF1alpha (401delta603) LCLL (HIF1A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    aa 401-603 deleted, removes the oxygen-dependent …
    Promoter
    CMV
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154846-plasmid-map-sequence-id-348286

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  12. pMEXneo-TrkC-TrkB-hybrid

    Plasmid
    #190205
    Purpose
    To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.
    Insert
    TrkC (NTRK3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The second IgG like domain and the extracellular …
    Promoter
    Moloney mouse sarcoma virus right LTR
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190205-plasmid-map-sequence-id-374467

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  13. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

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  15. pEMS1538

    Plasmid
    #29261
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29261-plasmid-map-sequence-id-13676

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Showing: 1581 - 1600 of 32215 results