We narrowed to 31,261 results for: son

Showing: 1581 - 1600 of 31261 results

Results per page:

Sort results by:

pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGs-Venus

Plasmid

#127346

Purpose

Expresses Venus constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Venus Fluorescent Protein

Expression

Mammalian

Promoter

CAGGS Promoter

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Tags

GFP

Expression

Mammalian

Promoter

human PGK promoter

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCWori cytochrome P450-BM3h 9D7

Plasmid

#61308

Purpose

Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

Depositor

Alan Jasanoff

Article

Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

Insert

Cytochrome P450-BM3 heme domain 9D7

Tags

6xHis

Expression

Bacterial

Mutation

T268A, I263A, T438V, A328G

Promoter

Tac

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

Plasmid

#172910

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2-ST

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-HyPer-DAAO-NES

Plasmid

#119164

Purpose

Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

HyPer-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGF196

Plasmid

#97005

Purpose

Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

LEMD2 (LEMD2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV delta 5

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Integrin-Alpha2-N-18

Plasmid

#55063

Depositor

Michael Davidson

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSU-araC-Cas9

Plasmid

#120423

Purpose

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64

Plasmid

#85422

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her_4D5-3 synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyC-kan

Plasmid

#84692

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-BH

Plasmid

#60515

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge