161,273 results

1. pDestTol2pA2_ubi:EGFP

   Plasmid

   #27323

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   ubiquitin:EGFP reporter
   

   Use

   Tol2 transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60226

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSyn-somBiPOLES-mCerulean

   Plasmid

   #154945

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CMV-Galpha-oA ONE-GO

   Plasmid

   #189732

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-oA ONE-GO
   GNAO (Gnao1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   I250 (silent mutation in YFP)

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Flamindo2

   Plasmid

   #73938

   Purpose

   Expresses biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A no ccDB (w48-1)

   Plasmid

   #17398

   Purpose

   Gateway entry 1A vector, no ccDB gene

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Use

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

   Plasmid

   #126580

   Purpose

   A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST_FUS

   Plasmid

   #26374

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMXS-IRES-Blast POLA1

    Plasmid

    #160807

    Purpose

    Express POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLA1 (POLA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon Optimized

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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