2,384 results

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pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Publication

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179072

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-2xPH(PLCd)

Expression

Yeast

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179073

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179059

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-HA KI

Plasmid

#183427

Purpose

CreON knock-in for GluA1-HA (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and HA donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and smFP HA donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo-FKBP KI

Plasmid

#183435

Purpose

CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and Halo-FKBP donor

Use

CRISPR

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor

Use

CRISPR

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and 4xGFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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LC3-mEYFP

Plasmid

#182865

Purpose

Autophagy Marker

Depositor

Salvatore Chiantia

Publication

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rat LC3 (Anxa3 Rat)

Expression

Mammalian

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3E LAP2b

Plasmid

#105978

Purpose

3' element for Gateway cloning, lamina-associated polypeptide 2

Depositor

Caren Norden

Publication

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Expression

Bacterial

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-rScn2a-3xV5-Syn-miRFP670

Plasmid

#182566

Purpose

For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Depositor

Zhe J. Liu

Publication

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

rat Scn2a gRNA and 3xV5 donor DNA
miRFP670

Use

AAV and CRISPR

Promoter

Syn and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRB144

Plasmid

#180175

Purpose

pCDNA3.1 Construct GluN1 A714L

Depositor

Richard Bergeron

Publication

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GluN1 (Grin1 Rat)

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Egr2ASRNA

Plasmid

#177737

Purpose

Egr2 AS-RNA lentiviral expression vector

Depositor

Nikolaos Tapinos

Publication

Martinez-Moreno et al Cell Rep. 2017 Aug 22;20(8):1950-1963. d

Insert

Egr2-AS-RNA

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV3 [N15/4R]

Plasmid

#177501

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)

Expression

Mammalian

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) WT

Plasmid

#134690

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) G361C

Plasmid

#134694

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

G361C, C145A, C217A, C249A, C290A, C338A, C387A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-jGCaMP7c variant 1513

Plasmid

#173159

Purpose

Expresses jGCaMP7c variant 1513 in astrocytes

Depositor

Hervé Chneiweiss, Vincent Vialou

Insert

jGCaMP7c variant 1513-WPRE

Use

AAV

Promoter

GFAP

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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