We narrowed to 4,881 results for: phy

1. pSC15

   Plasmid

   #104789

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g044580
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSC13

   Plasmid

   #104787

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g044580
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

   Plasmid

   #91935

   Purpose

   Bacterial expression of maize SUMO1a and inactive SCE1d

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1d(C94S)-Myc
   

   Use

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   C94S active site mutation and Truncation of ZmSUM…

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

   Plasmid

   #91933

   Purpose

   Bacterial expression of maize SUMO1a and inactive SCE1b

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1b(C94S)-Myc
   

   Use

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   C94S active site mutation and Truncation of ZmSUM…

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR-IAA8_no stop

   Plasmid

   #83874

   Purpose

   Gateway donor vector for IAA8 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR-IAA5_no stop

   Plasmid

   #83868

   Purpose

   Gateway donor vector for IAA5 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA5 (IAA5 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR-IAA3_no stop

   Plasmid

   #83863

   Purpose

   Gateway donor vector for IAA3 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA3 (SHY2 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR-IAA10_no stop

   Plasmid

   #83848

   Purpose

   Gateway donor vector for IAA10 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR-IAA4_no stop

   Plasmid

   #83866

   Purpose

   Gateway donor vector for IAA4 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA4 (ATAUX2-11 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR-IAA28_no stop

    Plasmid

    #83861

    Purpose

    Gateway donor vector for IAA28 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NP_568478.1 (IAA28 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR-IAA15_no stop

    Plasmid

    #83853

    Purpose

    Gateway donor vector for IAA15 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB1alpha1_SF (GB0106)

    Plasmid

    #68232

    Purpose

    Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTAct2 (GB0210)

    Plasmid

    #68191

    Purpose

    Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPMtb (GB0080)

    Plasmid

    #68167

    Purpose

    Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlMtb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPTctp (GB0083)

    Plasmid

    #68169

    Purpose

    Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlTCTP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5'UTRAct2 (GB0193)

    Plasmid

    #68178

    Purpose

    Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    5'UTR AtAct2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

    Plasmid

    #68213

    Purpose

    Transcriptional unit for Hygromycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hyg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGB1omega1_SF (GB0158)

    Plasmid

    #68242

    Purpose

    Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMyb12 (GB0075)

    Plasmid

    #68199

    Purpose

    Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Myb12 coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPAt2S3 (GB0029)

    Plasmid

    #68162

    Purpose

    Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    At2S3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUPD_Mini35s(-60,UTR) (GB0050)

    Plasmid

    #68176

    Purpose

    Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV Mini35S promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFRT-TODestMYC_RBPMS_KDR

    Plasmid

    #65667

    Purpose

    MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    introduced HindIII site to exchange FLAG-HA tag w…

    Promoter

    CMV, 2x Tet

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFRT-TODestFLAGHA_RBPMS_KDR_K100E

    Plasmid

    #65093

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 100 to glutamic acid (KDR backgrou…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFRT-TODestFLAGHA_RBPMS_KDR_F65A

    Plasmid

    #65092

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    change phenylalanine 65 to alanine (KDR backbone)

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFRT-TODestFLAGHA_RBPMS_K36E_R38E

    Plasmid

    #65095

    Purpose

    Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    change lysine 36 to glutamic acid and arginine 38…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFRT-TODestFLAGHA_RBPMS_R38Q

    Plasmid

    #65094

    Purpose

    Destination vector with RBPMS with R38Q mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed arginine 38 to glutamine

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFRT-TODestFLAGHA_RBPMS_KDR_K100A

    Plasmid

    #65663

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 100 to alanine (KDR background)

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A

    Plasmid

    #65658

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39A

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 36 to glutamic acid, arginine 38 t…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A

    Plasmid

    #65657

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, E39A

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 36 to alanine, arguing 38 to alani…

    Promoter

    CMV, 2x Tet

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

    Plasmid

    #65661

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed phenylalanine 27 to alanine (KDR backgrou…

    Promoter

    CMV, 2x Tet

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pVRc03_1244

    Plasmid

    #49731

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS03_1244
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Danio rerio cryaa V62T

    Plasmid

    #47630

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 62 to Threonine

    Promoter

    T7

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Danio rerio cryaa T147V

    Plasmid

    #47628

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Theronine 147 to Valine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Danio rerio cryaa C143S

    Plasmid

    #47619

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 143 to Serine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15b/GgVcl 1-851 A50I mutation

    Plasmid

    #46174

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 A50I mutation (talin binding domain mut…

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET30-GBF-E6N

    Plasmid

    #21699

    Depositor

    James Baleja

    Article

    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

    Insert

    Protein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
    

    Use

    Tags

    Protein G B1 domain

    Expression

    Bacterial

    Mutation

    GB1 (1-56aa) fusion tag with mutations at three p…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

38. FDXR KO-2A

    Plasmid

    #16614

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR knockout (FDXR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' targeting element

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

39. FDXR KO-1

    Plasmid

    #16613

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR knockout (FDXR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pBS CMV GAG

    Plasmid

    #234992

    Purpose

    For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    GAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted polymerase coding sequence

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge