We narrowed to 4,786 results for: Mos

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide2

   Plasmid

   #125401

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide4

   Plasmid

   #125399

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide3

   Plasmid

   #125398

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs4237150-guide1

   Plasmid

   #125397

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T1D/T2D-associated variant rs4237150 (GLIS3 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide1

   Plasmid

   #125393

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide1

   Plasmid

   #125400

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide2

   Plasmid

   #118178

   Purpose

   CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3

   Plasmid

   #118179

   Purpose

   CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide2

   Plasmid

   #118182

   Purpose

   CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118164

    Purpose

    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide1

    Plasmid

    #118181

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide1

    Plasmid

    #118173

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3

    Plasmid

    #118171

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2

    Plasmid

    #118174

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4

    Plasmid

    #118180

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide3

    Plasmid

    #118183

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide4

    Plasmid

    #118176

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4

    Plasmid

    #118172

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1

    Plasmid

    #118177

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1A-CD19.8H.8TM.28.BB.3z

    Plasmid

    #200672

    Purpose

    Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-EF1A-CD19.8H.28TM.BB.3z

    Plasmid

    #200675

    Purpose

    Modular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid

    #200673

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid

    #200677

    Purpose

    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

    Plasmid

    #192287

    Purpose

    Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid

    #200676

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS-GBX2-CDX124-Ef1a-mCherry-Neo

    Plasmid

    #192823

    Purpose

    Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMasterE

    Plasmid

    #165080

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    LIN28 (LIN28A Human)
    EGFP
    HSV TK
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTN186

    Plasmid

    #34890

    Depositor

    Mitsuyasu Hasebe

    Type

    Empty backbone

    Use

    Moss targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pECTO

    Plasmid

    #67683

    Purpose

    pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus

    Depositor

    Philippe Bouloc

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TrkC-GFP

    Plasmid

    #24094

    Depositor

    Moses Chao

    Insert

    TrkC (Ntrk3 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pKANAC3K

    Plasmid

    #221545

    Purpose

    Activation tagging for plant

    Depositor

    Keiichi Shimizu

    Type

    Empty backbone

    Use

    Activation tagging in plant

    Tags

    Expression

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Sept. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. p75 NTR

    Plasmid

    #24091

    Depositor

    Moses Chao

    Insert

    p75NTR (Ngfr Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. TrkA-RFP

    Plasmid

    #24093

    Depositor

    Moses Chao

    Insert

    TrkA (Ntrk1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    H7L, G21S, V23M, C32S, T39V

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Pfu-MBP

    Plasmid

    #215156

    Purpose

    Encodes a thermostable maltose binding protein

    Depositor

    Bhuvana Shanbhag, Travis Beddoe

    Insert

    Pfu-MBP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. cfSBFP2-N

    Plasmid

    #110633

    Purpose

    Cys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.

    Depositor

    Ikuo Wada

    Insert

    cfSBFP2
    

    Use

    Tags

    Expression

    Mutation

    Cys48Ser/Cys70Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOpen-THEAQlig

    Plasmid

    #165535

    Purpose

    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Taq DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pKS1466

    Plasmid

    #239516

    Purpose

    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScErg20p(F96W_N127W)-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MBP-YCup1

    Plasmid

    #215157

    Purpose

    Encodes a thermostable maltose binding protein fused to yeast copper metallothionein

    Depositor

    Bhuvana Shanbhag, Travis Beddoe

    Insert

    MBP-YCup1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKD3_mCherry

    Plasmid

    #187385

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pKD4_mCherry

    Plasmid

    #187386

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Use

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

50. pKM263-EcPPK

    Plasmid

    #38333

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge