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Showing: 4001 - 4050 of 5497 results

  1. pOpen-T4gene30

    Plasmid
    #165554
    Purpose
    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4-DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165554-plasmid-map-sequence-id-339904

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  2. pOpen-T5gene12/13

    Plasmid
    #165559
    Purpose
    Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T5 Exonuclease
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165559-plasmid-map-sequence-id-339952

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  3. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  5. pcDNA5-FRT-TO-AID-EGFP

    Plasmid
    #80076
    Purpose
    Mammalian expression vector for C-terminal AID-EGFP tagging
    Depositor
    Andrew Holland
    Insert
    AID-EGFP
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    80076-plasmid-map-sequence-id-150336

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  6. pcDNA5-FRT-TO-EGFP-AID

    Plasmid
    #80075
    Purpose
    Mammalian expression vector for N-terminal EGFP-AID tagging
    Depositor
    Andrew Holland
    Insert
    EGFP-AID
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    80075-plasmid-map-sequence-id-150337

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  11. pPD96_02

    Plasmid
    #1501
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1501-plasmid-map-sequence-id-112

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  12. pPD96_04

    Plasmid
    #1502
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1502-plasmid-map-sequence-id-113

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  13. pPD96_62

    Plasmid
    #1505
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2211, SV40NLS, gfpN, IVSR, gfpS65C, IVSS,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1505-plasmid-map-sequence-id-116

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  14. L4683

    Plasmid
    #1672
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, gfpN, IVSR, gfpGLA, IVSS, IVS…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1672-plasmid-map-sequence-id-282

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  15. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Strep
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  16. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

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  18. pGAE-UFO.664

    Plasmid
    #183471
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.664
    Depositor
    Andrea Cara
    Insert
    HIV envelope
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183471-plasmid-map-sequence-id-360575

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  19. pGAE-UFO.750

    Plasmid
    #183472
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.750
    Depositor
    Andrea Cara
    Insert
    HIV Envelope
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183472-plasmid-map-sequence-id-360574

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  21. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  22. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  23. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  25. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  26. CMV-myctagged MyoD

    Plasmid
    #8399
    Depositor
    Andrew Lassar
    Insert
    MyoD (Myod1 Mouse)
    Tags
    myc
    Expression
    Mammalian
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  27. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  28. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  29. pET28a_PTK2b

    Plasmid
    #224237
    Purpose
    Expression of human PTK2b Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b (PTK2B Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224237-plasmid-map-sequence-id-458420

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  30. pET11a_PTK2b_Kinase

    Plasmid
    #224242
    Purpose
    Expression of human PTK2b Kinase domain in E. coli
    Depositor
    Andrew Mesecar
    Insert
    ptk2b_kinase (PTK2B Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224242-plasmid-map-sequence-id-458378

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  31. PX385 Streptococcus pasteurianus Cas9

    Plasmid
    #68322
    Purpose
    Streptococcus pasteurianus Cas9
    Depositor
    Feng Zhang
    Insert
    SpaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68322-plasmid-map-sequence-id-119537

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  32. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

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  34. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  35. PX405 Neisseria meningitidis Cas9

    Plasmid
    #68702
    Purpose
    Neisseria meningitidis Cas9
    Depositor
    Feng Zhang
    Insert
    NmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. PX381 Filifactor alocis Cas9

    Plasmid
    #68318
    Purpose
    Filifactor alocis Cas9
    Depositor
    Feng Zhang
    Insert
    FaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68318-plasmid-map-sequence-id-119531

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  37. PX394 Neisseria cinerea Cas9

    Plasmid
    #68331
    Purpose
    Neisseria cinerea Cas9
    Depositor
    Feng Zhang
    Insert
    NcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68331-plasmid-map-sequence-id-119554

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  38. PX406 Pasteurella multocida Cas9

    Plasmid
    #68703
    Purpose
    Pasteurella multocida Cas9
    Depositor
    Feng Zhang
    Insert
    PmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. PX390 Flavobacterium columnare Cas9

    Plasmid
    #68327
    Purpose
    Flavobacterium columnare Cas9
    Depositor
    Feng Zhang
    Insert
    FcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68327-plasmid-map-sequence-id-119543

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  40. PRS_8-jGCaMP7b-P2A-mRuby3

    Plasmid
    #192595
    Purpose
    AAV-vector for red fluorescent labeling and green fluorescent calcium measurements from noradrenergic neurons
    Depositor
    Simon Wiegert
    Insert
    jGCaMP7b
    Use
    AAV
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    PRSX8
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192595-plasmid-map-sequence-id-379173

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  42. pMT-HA-STX4

    Plasmid
    #89311
    Purpose
    Expresses HA tagged syntaxin 4 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX4 (Syx4 Fly)
    Tags
    HA tag
    Expression
    Insect
    Promoter
    P-MT (Metallothionein)
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89311-plasmid-map-sequence-id-190169

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  44. PX395 Roseburia intestinalis Cas9

    Plasmid
    #68332
    Purpose
    Roseburia intestinalis Cas9
    Depositor
    Feng Zhang
    Insert
    RiCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68332-plasmid-map-sequence-id-119548

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  45. PX389 Fluviicola taffensis Cas9

    Plasmid
    #68326
    Purpose
    Fluviicola taffensis Cas9
    Depositor
    Feng Zhang
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68326-plasmid-map-sequence-id-119542

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  46. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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  47. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

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  48. PX387 Mycoplasma mobile Cas9

    Plasmid
    #68324
    Purpose
    Mycoplasma mobile Cas9
    Depositor
    Feng Zhang
    Insert
    MmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68324-plasmid-map-sequence-id-119539

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  49. PX392 Azospirillum B510 Cas9

    Plasmid
    #68329
    Purpose
    Azospirillum B510 Cas9
    Depositor
    Feng Zhang
    Insert
    AzoCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68329-plasmid-map-sequence-id-119551

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Showing: 4001 - 4050 of 5497 results