We narrowed to 3,249 results for: cat.3

1. pcDNA3 RL3m6L2

   Plasmid

   #109366

   Purpose

   Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
   

   Tags

   1D4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rb-379-928Δ703-737-pGex2T

   Plasmid

   #21933

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

   Insert

   Rb (RB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928D703-737 o…

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3b(1454/3172)P2P-mut

   Plasmid

   #21405

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   hypoxia response element mutated

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. Rb-379-928Δ738-775-pGex2T

   Plasmid

   #21783

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

   Insert

   Rb (RB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928D737-774 o…

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGADT7-ε

   Plasmid

   #197261

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-4 ε (AP4E1 Human)
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitutions in codons 905, 1129 and 1137

   Promoter

   ADH1

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4 3xFLAG Halo G-Linker nsp1

   Plasmid

   #175426

   Purpose

   Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   G-linker nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   G-linker in place of the RNase destabilization do…

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4 3xFLAG Halo R119A K120A nsp1

   Plasmid

   #175428

   Purpose

   Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 R119A K120A
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants R119A K120A

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 3xFLAG Halo E36A E37A nsp1

   Plasmid

   #175429

   Purpose

   Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 E36A E37A
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminal mutants E36A E37A

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDTCendoNA2

   Plasmid

   #174732

   Purpose

   Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

   Depositor

   Jukka Finne

   Article

   Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

   Insert

   DT386C
   

   Expression

   Bacterial

   Mutation

   Deleted amino acids 412-560 (the receptor binding…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid

    #139459

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108500

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108504

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK-VIM270-gCM

    Plasmid

    #215868

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4 3xFLAG Halo delta 122-130 nsp1

    Plasmid

    #175425

    Purpose

    Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 122-130 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psiCHECK-VIM270-pCM

    Plasmid

    #215869

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

    Plasmid

    #175427

    Purpose

    Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E55A E57A K58A
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E55A E57A K58A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

    Plasmid

    #108503

    Purpose

    inducible expression of vsv-INCENP d543-746-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746 (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha helix

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP

    Plasmid

    #108505

    Purpose

    inducible expression of vsv-INCENP 8KR-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    K548R, K563R, K602R, K609R, K614R, K622R, K630R, …

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP

    Plasmid

    #108506

    Purpose

    inducible expression of vsv-INCENP 8KQ-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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