We narrowed to 4,333 results for: chm

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymYPET-CaURA3

Plasmid

#118460

Purpose

Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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PX458 pSp Cas9 Rhino1 tagA

Plasmid

#211627

Purpose

Cas9 Rhino1 tagA

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

RHNO tagA

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDM_noro_A2_lacZA

Plasmid

#118819

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A2

Expression

Bacterial

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-TEV-pro-conA

Plasmid

#159527

Purpose

Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

Depositor

Joshua Mylne

Article

Nonis et al bioRxiv. 2020.

Insert

Canavalia ensiformis pro-concanavalin A

Tags

Six-Histidine tag and tobacco etch virus (TEV) pr…

Expression

Bacterial

Promoter

T5

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-RhoH

Plasmid

#23230

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoH (RHOH Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
JP1520 PIK3CA E545K HA

Plasmid

#14571

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA E545K (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-12

Plasmid

#119991

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG824

Plasmid

#105401

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

March 27, 2018

Availability

Academic Institutions and Nonprofits only

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pFUS_B10

Plasmid

#31027

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-HA-mAVO3 (pRL62-3)

Plasmid

#39210

Depositor

Michael Hall

Article

Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Ep

Insert

mAVO3 (Rictor Mouse)

Tags

HA

Expression

Mammalian

Mutation

G5S and I15V

Promoter

CMV

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-ytdTomato-CaURA3

Plasmid

#118461

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHD1

Plasmid

#30974

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRDA_450

Plasmid

#179094

Purpose

nuclease Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpRY

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymVenus-CaURA3

Plasmid

#118459

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection. *An updated version of this plasmid is available: #168058 pFA6a-link-ymVenus-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDC20

Plasmid

#131366

Purpose

expression of MEG-3 (aa1-862) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-862) (meg-3 Nematode)

Tags

His

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNG1

Plasmid

#30985

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG1

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-CMV-Puro

Plasmid

#127169

Purpose

PCR template for CMV-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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