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PE8c

Plasmid

#257699

Purpose

in vitro transcription plasmid for PE8c

Depositor

David Liu

Article

Tao et al Nat Biotechnol. 2026 May 21. doi: 10.103

Insert

PE8c prime editor

Use

Template for in vitro transcription

Available Since

June 3, 2026

Availability

Academic Institutions and Nonprofits only

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-100bp

Plasmid

#16294

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -88 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1

Plasmid

#215030

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTALETF_v2 (NG)

Plasmid

#32186

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NG for 0.5 repeat

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pTALETF_v2 (HD)

Plasmid

#32188

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, HD for 0.5 repeat

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pU6T7G

Plasmid

#71463

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMax_mPlum

Plasmid

#177830

Purpose

Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Oct4-TK-HMS

Plasmid

#62351

Purpose

Mouse Oct4 targeting vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

TK-Hyg-24xMS2

Use

Mouse Targeting

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMax_ScaI_BFP

Plasmid

#177824

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP

Expression

Mammalian

Mutation

5' inserted ScaI RE cut site

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pLM-AMA18.0 dCas9-VPR

Plasmid

#138945

Purpose

Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Article

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Promoter

p40S (AN0465) Aspergillus nidulans

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO- FOS

Plasmid

#125598

Purpose

doxycycline-inducible expression of human FOS in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Fos proto-oncogene, AP-1 transcription factor subunit (FOS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

Plasmid

#62345

Purpose

Expresses MCP-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

MCP-tdiRFP670

Tags

HA and SV40 NLS

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMycMyt1-7zf/IRES-Red

Plasmid

#22652

Depositor

Lynn Hudson

Article

Nielsen et al Mol Cell Neurosci. 2004 Jan . 25(1):111-

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-mCherry

Plasmid

#127538

Purpose

Expresses human SOX2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

Plasmid

#39228

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Mutation

C4762A

Promoter

T7

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pAD12

Plasmid

#34832

Depositor

Cynthia Kenyon

Article

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

None

Use

RNAi

Expression

Bacterial

Available Since

June 11, 2012

Availability

Academic Institutions and Nonprofits only