We narrowed to 4,949 results for: mcs

1. pENTR SD Age HsSAS-6

   Plasmid

   #46381

   Purpose

   For expression of C-terminal fusions.

   Depositor

   Pierre Gonczy

   Article

   Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

   Insert

   SAS-6 (SASS6 Human)
   

   Use

   Entry vector

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-EGFAB(H306Y)

   Plasmid

   #25771

   Depositor

   Johann Deisenhofer

   Article

   McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

   Insert

   LDL receptor EGFAB (LDLR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   His 306 to Tyr

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

3. S12.1_Empty_Bridge

   Plasmid

   #127670

   Purpose

   Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

   Plasmid

   #21800

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   PSMD14 (Psmd14 Mouse)
   

   Tags

   GST and TEV

   Expression

   Mammalian

   Available Since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLR2070

   Plasmid

   #28063

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains N496D point mutation in FokI domain, as …

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLR2068

   Plasmid

   #28062

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains H537R point mutation in FokI domain, as …

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV2A-T

   Plasmid

   #153022

   Purpose

   Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

   Depositor

   Vito Valiante

   Article

   Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

   Plasmid

   #182355

   Purpose

   mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL1 Trx-Bx

   Plasmid

   #106290

   Purpose

   Expresses Trx-Bx in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   Trx-BX
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid

    #182347

    Purpose

    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3495 pcDNA3 Bid p15

    Plasmid

    #8780

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Expression

    Mammalian

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. S12.2_Bridge + Target

    Plasmid

    #127671

    Purpose

    Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLLX13

    Plasmid

    #79825

    Purpose

    Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

    Depositor

    Stephen Lory, Cammie Lesser

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Yeast and bacteria shuttle vector, contains origi…

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-TPI_PTC160-xrRNA-4H

    Plasmid

    #108369

    Purpose

    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-TPI_WT-xrRNA-4H

    Plasmid

    #108368

    Purpose

    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag Snail 8SA

    Plasmid

    #16222

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pENTR Age HsSAS-6 ST

    Plasmid

    #46382

    Purpose

    For expression of N-terminal fusions.

    Depositor

    Pierre Gonczy

    Article

    Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

    Insert

    SAS-6 (SASS6 Human)
    

    Use

    Entry vector

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-merlin 1-339

    Plasmid

    #168128

    Purpose

    Bacterial expression of human Nf2/merlin 1-339

    Depositor

    Tina Izard

    Article

    Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

    Insert

    Merlin (NF2 Human)
    

    Tags

    Glutathione S-transferase (GST)

    Expression

    Bacterial

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HGXPRT

    Plasmid

    #64201

    Purpose

    Expression of Plasmodium falciparum HGXPRT

    Depositor

    Vern Schramm

    Article

    Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

    Insert

    Hypoxanthine guanine xanthine phosphoribosyltransferase
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only