We narrowed to 1,704 results for: pyogenes Cas9

1. p2702-AGER-gRNA1

   Plasmid

   #216471

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2703-AGER-gRNA2

   Plasmid

   #216472

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2704-AGER-gRNA3

   Plasmid

   #216473

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MultiMate-HITI-2c ACTB

   Plasmid

   #206267

   Purpose

   All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-MMETn-del-gRNA2

   Plasmid

   #185007

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMETn-del-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-IAP-del-gRNA1

   Plasmid

   #185002

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA1 (Akp3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-MMERVK9C-del-gRNA1

   Plasmid

   #185004

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMERVK9C-del-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-MMETn-del-gRNA1

   Plasmid

   #185006

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMETn-del-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX458-MMERVK9C-del-gRNA2

   Plasmid

   #185005

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   MMERVK9C-del-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX458-IAP-del-gRNA2

    Plasmid

    #185003

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    IAP-del-gRNA2 (Akp3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-PE2-His (IK1822)

    Plasmid

    #170103

    Purpose

    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

    Depositor

    Keith Joung

    Article

    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    

    Use

    CRISPR

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Bacteria codon-optimized nSpCas9 (H840A) & M-…

    Promoter

    T7

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-p53DD

    Plasmid

    #196582

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE2-P2A-BSD

    Plasmid

    #174819

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-BSD
    

    Use

    Tags

    P2A-BSD and SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_PE7-2A-BSD

    Plasmid

    #234073

    Purpose

    For lentiviral expression of PE7 with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PE7
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PEmax-P2A-hP53DD

    Plasmid

    #214084

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax-P2A-hP53DD (TP53 Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Use

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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