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pMYT034

Plasmid

#180687

Purpose

Encodes LYS2 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

LYS2

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT033

Plasmid

#180686

Purpose

Encodes MET17 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

MET17

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT032

Plasmid

#180685

Purpose

Encodes TRP1 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

TRP1

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT031

Plasmid

#180684

Purpose

Encodes HIS3 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

HIS3

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT030

Plasmid

#180683

Purpose

Encodes LEU2 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

LEU2

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT029

Plasmid

#180682

Purpose

Encodes URA3 as a yeast Vector selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

URA3

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT028

Plasmid

#180681

Purpose

Encodes tPDC1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

tPDC1

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT027

Plasmid

#180680

Purpose

Encodes tTDH2 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

tTDH2

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT026

Plasmid

#180679

Purpose

Encodes tTEF1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

tTEF1

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT025

Plasmid

#180678

Purpose

Encodes tTDH3 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

tTDH3

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCyc-Bem1

Plasmid

#66573

Purpose

Express an additional copy of Bem1, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Bem1

Expression

Yeast

Mutation

F99I*(see below)

Promoter

pCyc1

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1509

Plasmid

#62363

Purpose

pFA6a BirA-heh2 linker-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-heh2-Ssh1

Expression

Yeast

Promoter

Gal1

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMal672H

Plasmid

#25817

Depositor

Cynthia Wolberger

Article

Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31.

Insert

Rap1(672-827) (RAP1 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 26, 2010

Availability

Academic Institutions and Nonprofits only
pYBS369

Plasmid

#18885

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste7 (STE7 Budding Yeast)

Expression

Yeast

Mutation

amino terminal 172 aa of Ste7

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pYBS370

Plasmid

#18886

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste7 (STE7 Budding Yeast)

Expression

Yeast

Mutation

kinase domain of C-terminal 344 residues

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pHIS3p:CloverGFP-Tub1+3'UTR::URA3

Plasmid

#50636

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

CloverGFP-Tub1

Use

Yeast expression

Tags

CloverGFP

Promoter

HIS3p

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW LAMP1Sig-mCherry

Plasmid

#239556

Purpose

Expression and lysosomal localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pYCTK034

Plasmid

#176738

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

bar1Sc

Use

Synthetic Biology

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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