164,826 results

1. pDONR221_SLC22A15

   Plasmid

   #132011

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A15 (SLC22A15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. SSTR2-Tango

   Plasmid

   #66503

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   SSTR2 (SSTR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. hsKIF5B(1-560)-HaloTag-FRB

   Plasmid

   #213899

   Purpose

   Imaging of KIF5B fused to HaloTag

   Depositor

   Lukas Kapitein

   Insert

   hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Venus1-S727A-STAT3

   Plasmid

   #123174

   Purpose

   Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   S727A substitution

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. tsPurple chromoprotein

   Plasmid

   #117848

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFW1000

   Plasmid

   #224210

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus1-Y705F/S727A-STAT3

   Plasmid

   #123176

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-mDlx-ChR2-mCherry-Fishell-3

   Plasmid

   #83898

   Purpose

   ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   ChR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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