161,531 results

1. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEM-Cas9HF1

   Plasmid

   #89961

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9HF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-CDK12

   Plasmid

   #116723

   Purpose

   Lentiviral expression of CDK12

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   CDK12 (CDK12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-cT3G-cERP2-HDAC2

   Plasmid

   #222553

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HDAC2 (HDAC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEVY-L

   Plasmid

   #51226

   Purpose

   bi-directional expression vector for yeast, constitutively active, LEU2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD/ADH1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pINDUCER dCas9-TET1CDMut

   Plasmid

   #101920

   Purpose

   The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD Mut
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutated TET1 catalytic domain

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-RAB10 T23N

   Plasmid

   #130885

   Purpose

   Expresses human RAB10 T23N mutant with N-terminal GFP tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   RAB10 (RAB10 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgOpti

   Plasmid

   #85681

   Purpose

   Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

   Depositor

   Eric Lander, David Sabatini

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. phage-ubc-nls-ha-tdPCP-gfp

   Plasmid

   #40650

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdPCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA SF-HERC2 F4

    Plasmid

    #55787

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only