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Showing: 1621 - 1640 of 13372 results

  2. DT034A

    Plasmid
    #159395
    Purpose
    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…
    Expression
    Bacterial
    Mutation
    Promoter
    J23117 and pLux promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159395-plasmid-map-sequence-id-393061

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  4. pAA3

    Plasmid
    #154163
    Purpose
    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    20 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154163-plasmid-map-sequence-id-309398

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  5. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid
    #181858
    Purpose
    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-EKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181858-plasmid-map-sequence-id-363410

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  8. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

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  12. pENTR4 HopU1

    Plasmid
    #228395
    Purpose
    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Article
    Simranjit et al bioRxiv 2023.09.25.558804
    Insert
    HopU1
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Alanine inserted after start Methionine due to Nc…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    228395-plasmid-map-sequence-id-462965

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  13. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  15. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  20. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid
    #166415
    Purpose
    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Article
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Acytostelium subglobosum PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166415-plasmid-map-sequence-id-329462

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Showing: 1621 - 1640 of 13372 results