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Showing: 1621 - 1640 of 8297 results

  1. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Use
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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  5. pDONR P2R-P3-V5-6xHis

    Plasmid
    #48347
    Purpose
    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    V5 and 6xHis epitope tags cassette
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Contains a stop codon at the end of the epitope t…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48347-plasmid-map-sequence-id-70558

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  6. pGLTR-S-PURO

    Plasmid
    #58244
    Purpose
    GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and no
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58244-plasmid-map-sequence-id-89655

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  10. S113

    Plasmid
    #174551
    Purpose
    Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)
    Depositor
    Bruno Correia
    Article
    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:
    Insert
    mdm2 and designed protein LD6 (MDM2 )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174551-plasmid-map-sequence-id-343593

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  11. CMV-10xMS2 no wild type

    Plasmid
    #158204
    Purpose
    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of MS2 without the WT sequence from oligo library experiment
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158204-plasmid-map-sequence-id-311816

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  12. CMV-10xQb no wild type

    Plasmid
    #158205
    Purpose
    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158205-plasmid-map-sequence-id-311817

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  15. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

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  19. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  20. pET15b MOSPD2 RD/LD [282-490]

    Plasmid
    #186470
    Purpose
    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Tags
    6HIS
    Expression
    Bacterial
    Mutation
    MSP domain [282-490]
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    186470-plasmid-map-sequence-id-367335

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Showing: 1621 - 1640 of 8297 results