Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 30,611 results for: PLE

Showing: 2431 - 2460 of 30611 results

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pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo

Plasmid

#250259

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(T343V)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

T343V

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2724 - pLenti-X1-EF1A-HA-FBXO31(∆F-box)-PGK-Neo

Plasmid

#250256

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2725 - pLenti-X1-EF1A-HA-FBXO31(Y309A)-PGK-Neo

Plasmid

#250257

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(Y309A)).

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Y309A

Promoter

EF-1a

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

Plasmid

#218558

Purpose

Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-MLLx3-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

Plasmid

#236241

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV1.2 (CACNA1C Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) Triple RBD

Plasmid

#212412

Purpose

Encodes SARS-CoV-2 spike K417T,E484K,N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417T,E484K,N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417T,E484K,N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

Plasmid

#211621

Purpose

Rhno1 T52A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

T52A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

Plasmid

#211620

Purpose

Rhno1 S51A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S51A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro

Plasmid

#211624

Purpose

Rhno1 S163A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S163A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro

Plasmid

#211623

Purpose

Rhno1 S162A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S162A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro

Plasmid

#211625

Purpose

Rhno1 S164A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S164A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro

Plasmid

#211626

Purpose

Rhno1 S178A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S178A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

Plasmid

#211815

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and puromycin selection gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter, U6 promoter, and hPGK promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

Plasmid

#204724

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166134

Purpose

This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting firefly luciferase gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

Plasmid

#167199

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R S364L

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

Plasmid

#167200

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R D421N

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

Plasmid

#167198

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R E294K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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