We narrowed to 9,182 results for: Pol;

1. pAAV-CaMKII-Ace-mNeon2

   Plasmid

   #195526

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   CaMKII

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-DIO-VARNAM2-Kv2.1PR

   Plasmid

   #195533

   Purpose

   Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CAG

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV-Tlr4

   Plasmid

   #27148

   Depositor

   Bruce Beutler

   Article

   Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

   Insert

   toll-like receptor 4 (Tlr4 Mouse)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Native signal peptide sequence (original amino ac…

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

   Plasmid

   #195536

   Purpose

   Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CAG

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKII-VARNAM2-Kv2.1PR

   Plasmid

   #195530

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   CaMKII

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-pAceR-Kv2.1PR

   Plasmid

   #195525

   Purpose

   Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAceR-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM 78E, 81D, 92N; SI linker

   Promoter

   Synapsin

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-MS2V5-PABPC1 M161A/D165K

   Plasmid

   #65809

   Purpose

   expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   PABPC1 (PABPC3 Human)
   

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   M161A/D165K

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV2/9-CW3SL-mCherry-DrTrkB

    Plasmid

    #184002

    Purpose

    AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    AAV

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRE-Hsp70BbCas9_1.2

    Plasmid

    #190796

    Purpose

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Expression

    Insect

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGWB122

    Plasmid

    #68576

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

    Plasmid

    #65810

    Purpose

    expression of PABPC1-MS2 fusion, double mutant.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC4 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    MLLEmut M161A/D165K

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CaMKII-pAceR-Kv2.1PR

    Plasmid

    #195531

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CaMKII

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dr-EGFR

    Plasmid

    #184004

    Purpose

    Dr-EGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-EGFR (EGFR Human)
    

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

    Plasmid

    #83453

    Purpose

    Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    S4144D

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMpGWB119

    Plasmid

    #68573

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

    Plasmid

    #83461

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, R4113A

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMpGWB321

    Plasmid

    #68649

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pMpGWB121

    Plasmid

    #68575

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB222

    Plasmid

    #68613

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMpGWB219

    Plasmid

    #68610

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMpGWB221

    Plasmid

    #68612

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMpGWB319

    Plasmid

    #68647

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)

    Plasmid

    #83465

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only