We narrowed to 17,506 results for: Emb
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Plasmid#214137PurposeInsect cell expression of the human 20SCP proteasome assembly chaperonesDepositorInsertsExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pTH753-CEN-RLuc/min346maxCFLuc
Plasmid#38219DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 346 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
STT3A pmRFP (663)
Plasmid#61997Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2
Plasmid#236246PurposeEndogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membraneDepositorInsertgRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
UseAAVTagsmTagBFP2PromoterU6 and human Synapsin1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)
Plasmid#229845PurposeA CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.DepositorInsertLamin nanobody
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGM238
Plasmid#220178PurposeExpresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)MutationK78E (NACA) + K43B (BTF3)Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
STT3A pEGFP-N2 (1051)
Plasmid#62025Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-Mut6E
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
TagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-EGFP-TEV
Plasmid#105810PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsEGFP-TEVExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-FLAG
Plasmid#105811PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-mCherry-TEV
Plasmid#105808PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsmCherry-TEVExpressionMammalianAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-FLAG-TEV
Plasmid#105807PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192239Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3eDepositorInserteIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
ExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
ExpressionMammalianAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-TEV-FLAG
Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeIF4E
Plasmid#79258PurposeExpresses EGFP-tagged DmeIF4E in S2 cellsDepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hRSK3(RPS6KA2)_K91A_K444A
Plasmid#180385Purposelentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activityDepositorInsertRPS6KA2_K91A/K444A (RPS6KA2 Human)
UseLentiviralExpressionMammalianMutationK91A/K444APromoterCMVAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-TEV-EGFP
Plasmid#105814PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-EGFPExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH752-CEN-RLuc/min103maxCFLuc
Plasmid#38218DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 103 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH751-CEN-RLuc/min53maxCFLuc
Plasmid#38217DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 53 codons of the original FLuc gene wer…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRAF-V600E-IRES-mCherry
Plasmid#221026PurposeFluorescent reporter for expressing a segment of BRAF-V600E CDSDepositorAvailable SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHLsec-dasTMPRSS2
Plasmid#207850PurposeExpresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cellsDepositorInserttransmembrane serine protease 2 (TMPRSS2 Human)
TagsAviTag, 8xHIS tagExpressionMammalianMutationdeleted amino acid 1-108, S251D R252D Q253D S254D…PromoterCAGAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo PTEN sgRNA5
Plasmid#233246PurposeKnock out of murine PTENDepositorInsertPTEN (Pten Mouse)
UseLentiviralAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo sgITGA5_5
Plasmid#233253PurposeKnock out of murine ITGA5DepositorInsertITGA5 (Itga5 Mouse)
UseLentiviralAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo sgITGA5_4
Plasmid#233252PurposeKnock out of murine ITGA5DepositorInsertITGA5 (Itga5 Mouse)
UseLentiviralAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo sgITGA5_1
Plasmid#233251PurposeKnock out of murine ITGA5DepositorInsertITGA5 (Itga5 Mouse)
UseLentiviralAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo sgAGAP1_3
Plasmid#233248PurposeKnock out of murine AGAP1DepositorInsertAGAP1 (Agap1 Mouse)
UseLentiviralAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo PTEN sgRNA2
Plasmid#233245PurposeKnock out of murine PTENDepositorInsertPTEN (Pten Mouse)
UseLentiviralAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 Neo sgAGAP1_2
Plasmid#233247PurposeKnock out of murine AGAP1DepositorInsertAGAP1 (Agap1 Mouse)
UseLentiviralAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-636_pGEX6P1
Plasmid#209789PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
TagsGSTExpressionBacterialMutationaa 544-636 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k sgRNA-2 PX459 plasmid
Plasmid#192256Purposeexpressing sgRNA-2 targeting eIF3k locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k sgRNA-1 PX459 plasmid
Plasmid#192255Purposeexpressing sgRNA-1 targeting eIF3k locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f sgRNA-1 PX459 plasmid
Plasmid#192253Purposeexpressing sgRNA-1 targeting eIF3f locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f sgRNA-2 PX459 plasmid
Plasmid#192254Purposeexpressing sgRNA-2 targeting eIF3f locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only