We narrowed to 13,013 results for: Hal
-
Plasmid#121794PurposepMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectorsDepositorInsertAID + WPRE
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KJ201: pMVP (R4-R3) osTir1 open
Plasmid#121743PurposepMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1 (open)
UseGateway Cloning and Synthetic BiologyTags9x myc epitope tagAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO801: pMVP (L5-L4) Hygro-P2A
Plasmid#121718PurposepMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.DepositorInsertHygro-P2A (open)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_HLF_2
Plasmid#86302PurposeEncodes gRNA for 3' target of human HLFDepositorInsertgRNA against HLF (HLF Human)
UseCRISPRAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HLF
Plasmid#86262PurposeDonor vector for 3' FLAG tag of human HLFDepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_HLF_1
Plasmid#86301PurposeEncodes gRNA for 3' target of human HLFDepositorInsertgRNA against HLF (HLF Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP34∆sfGFP
Plasmid#247211PurposeExpresses a Pepper RNA aptamer (tDF30ppr) from a T7 promoter. Suitable for cell-free expression.DepositorInsertDimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
UseSynthetic Biology; E. coli-based cell-free expres…ExpressionBacterialMutationTwo mutations were made to the second Pepper unit…PromoterT7Available SinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFP35∆sfGFP
Plasmid#247212PurposeExpresses a Pepper RNA aptamer (DF30ppr) from a T7 promoter. Suitable for cell-free expression.DepositorInsertDimeric Pepper RNA aptamer (with F30 scaffold)
UseSynthetic Biology; E. coli-based cell-free expres…ExpressionBacterialMutationTwo mutations were made to the second Pepper unit…PromoterT7Available SinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targeting tdTomato
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP
Plasmid#184819PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.DepositorInsertQF2-pA-he1.1:YFP
UseCRISPR; Backbone for cloning of homology arms (fo…Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
ExpressionYeastMutationFour Arg to Ala mutations in zinc finger backboneAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-160
Plasmid#219414PurposeEstradiol-inducible expression of 13-6 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
ExpressionYeastAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
Tags6xHis, GFP11, and R9ExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backbonePromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898
Plasmid#105635PurposeExpresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmidDepositorExpressionBacterialMutationMutations: F11S, G12A, V132K, C133V, A135KAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014
Plasmid#105637PurposeExpresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmidDepositorExpressionBacterialMutationMutations: F11S, G12A, V132K, C133V, A135KPromoterT7Available SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8
Plasmid#175796Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomersDepositorInserttcTRP24sub8
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_SS
Plasmid#175798Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'DepositorInserttcTRP24sub8_SS
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub8_SH2sub6
Plasmid#175799Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2DepositorInserttcTRP24sub8_SH2sub6
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP9sub3
Plasmid#175794Purposede novo thick circular tandem repeat protein trimer formed from 3-repeat monomersDepositorInserttcTRP9sub3
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
tcTRP24sub6
Plasmid#175795Purposede novo thick circular tandem repeat protein tetramer formed from 6-repeat monomersDepositorInserttcTRP24sub6
Tags6xHisExpressionBacterialAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
4LNDD-pCDNA3.1(+)
Plasmid#172227PurposeExpresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis and FlagExpressionMammalianPromoterCMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddtoxA-C
Plasmid#171724PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddtoxA-C
Plasmid#171728PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddtoxA-C
Plasmid#171732PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddtoxA-N
Plasmid#171725PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddtoxA-N
Plasmid#171729PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddtoxA-N
Plasmid#171733PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168168PurposeExpression of YFP-CaCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
TagsYFPExpressionWormMutationE497K and C566DAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168171PurposeExpression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
TagsYFPExpressionWormAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
TagsGFPExpressionMammalianMutationH112A and RNAi resistanceAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA44
Plasmid#158522PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.DepositorAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA18
Plasmid#158496PurposeIn planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.DepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only