We narrowed to 2,675 results for: CCS
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Plasmid#127090PurposeCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activationDepositorHas ServiceAAV PHP.eBInsertChannelrhodopsin-2
UseAAVTagseYFP (C terminal on insert)ExpressionMammalianMutationHumanized ChR2 gene with histidine 134 changed to…PromoterCAGAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eGFP-PC1-3HA
Plasmid#108406PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.DepositorAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNluc-sgControl
Plasmid#208383PurposeThis sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.DepositorInsertsgControl (TP53BP1 intron) (Trp53bp1 Mouse)
UseLentiviral and LuciferaseExpressionMammalianMutationWTAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-cAMPr
Plasmid#160723PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.DepositorInsertS2-cAMPr
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI
Plasmid#126480PurposeExpresses Cas9-GSAlinker-TdT in mammalian cells.DepositorInsertsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-CEBPD
Plasmid#192897PurposeBarcoded piggybac transposon vector with Dox-inducible expression of CEBPDDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1 3SA
Plasmid#121489PurposeExpression of mouse PD-L1 3SADepositorInsertPD-L1 3SA
UseLentiviralTagsFlagExpressionMammalianMutationS175A, T179A, S183APromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)
Plasmid#224123PurposeMammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)DepositorInsertPE7-mutant (Q20A_Y23A_Y24F_F35A)
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationQ20A_Y23A_Y24F_F35A in La(SSB)1-194PromoterCMVAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT
Plasmid#132667PurposeExpresses hypoxia-inducible Cas9 and TdT in mammalian cells.DepositorInsertCas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)
ExpressionMammalianPromoter4xHRE_YB TATAAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NL-BCL2L1
Plasmid#113458PurposeNanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)DepositorInsertBCL2 like 1 (BCL2L1 Human)
UseLuciferaseTagscmyc-NanoLucExpressionMammalianPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)
Plasmid#208395PurposeTo generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralExpressionMammalianMutationDB1delta/DB2deltaAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
Plasmid#85800PurposedonorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BneonDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingMutationhomology arms for knock-in into first intronAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-ExRaiAKAR
Plasmid#160724PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.DepositorInsertS3-ExRaiAKAR
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S4-ExRaiCKAR
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …Available SinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-cT3G-cERP2-HOPX
Plasmid#192923PurposeBarcoded piggybac transposon vector with Dox-inducible expression of HOPXDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AF20-double-tag
Plasmid#108389PurposeExpress human PC1DepositorInsertPKD1 (PKD1 Human)
TagseGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5ExpressionMammalianPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97308PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb HMEJ donor
UseAAV and Mouse TargetingExpressionMammalianPromoterU6, EF1aAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
Plasmid#136580Purposemammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)DepositorInsertNRF2 (C506S, Leuzip mut) (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationThe C506S mutation (C513S in the actual sequence …Available SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only