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Showing: 1621 - 1640 of 1686 results

  1. pQP-2376

    Plasmid
    #138974
    Purpose
    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
    Depositor
    Vladislav Verkhusha
    Article
    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:
    Insert
    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138974-plasmid-map-sequence-id-291767

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  3. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid
    #49062
    Purpose
    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    N-terminal deletion of amino acids 13-222 in hNKC…
    Promoter
    CMV
    Available since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49062-plasmid-map-sequence-id-72184

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  10. pcDNA3-ABKAR

    Plasmid
    #140051
    Purpose
    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.
    Depositor
    Jin Zhang
    Article
    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4
    Insert
    ABKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140051-plasmid-map-sequence-id-307466

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  11. pcDNA3-ER-AKAR4

    Plasmid
    #64733
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    AKAR4
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64733-plasmid-map-sequence-id-109352

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  13. pYG026

    Plasmid
    #65328
    Purpose
    reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    ccdB
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    65328-plasmid-map-sequence-id-110561

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  17. 1056H

    Plasmid
    #183135
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183135-plasmid-map-sequence-id-362097

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  18. 1056J

    Plasmid
    #183136
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183136-plasmid-map-sequence-id-362100

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  19. 1056K

    Plasmid
    #183137
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183137-plasmid-map-sequence-id-362101

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  20. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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Showing: 1621 - 1640 of 1686 results