We narrowed to 14,123 results for: cas9 genes
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Plasmid#202009PurposeMoclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSPDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo level 1 vectorTagsGS linkersPromoterCmYLCVAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVCERC-5 Pvan-dcas9 (Sth3)
Plasmid#133319Purposeexpresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedPromotervanillateAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-TRE-dCas9-EGFP
Plasmid#62347PurposePlasmid for tet-inducible expression of dCas9-EGFP in mammalian cellsDepositorInsertdCas9-EGFP
UseCRISPRTagsHA and SV40 NLSExpressionMammalianPromoterTREAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC5-dual-dCas9VP48-sgTetO
Plasmid#48237PurposeDual expression construct expressing both dCas9VP48 and sgTetO from separate promotersDepositorInsertdCas9VP48 and sgTetO
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)Available SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-tomato-TSC2-sgRNA
Plasmid#196195PurposeEditing human TSC2 locusDepositorInsertTSC2 (TSC2 Human)
UseCRISPRAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-EGFP-L2
Plasmid#141046PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n-2A-EGFP
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedPromoterxyloseAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNA500
Plasmid#149583Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …Available SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-VPR-WT CjCas9 V-WT
Plasmid#169914PurposeExpression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.DepositorInsertCjCas9
UseCRISPRTags2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)ExpressionMammalianPromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRPromoterxyloseAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC12)_Psyn-sgRNArodA
Plasmid#149661Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC12), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTet-OnAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAftsI
Plasmid#149590Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN-CMV-Cas9-V5-WPRE
Plasmid#87904PurposeTo produce lentiviral vector for gene editingDepositorInsertCas9-V5
UseLentiviralTagsV5Mutationhuman codon-optimizedPromoterCMVAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
Plasmid#149594Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only