We narrowed to 4,949 results for: mcs

1. Empty Anchor Control

   Plasmid

   #127665

   Purpose

   Encodes for puromycin resistance expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   Puro
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pmyo3::tag:sl1(G16C)

   Plasmid

   #79003

   Purpose

   Expression of exogenous SL1 in C. elegans muscle

   Depositor

   Ge Zhan, Xiao Liu

   Article

   Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

   Insert

   sls-1.2 (sls-1.2 Nematode)
   

   Tags

   illumina-adapter

   Expression

   Worm

   Mutation

   G16C

   Promoter

   myo-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP Snail 8SA

   Plasmid

   #16229

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMM2-hPMCA2x/b

   Plasmid

   #47582

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2x/b (ATP2B2 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA_GFP_mTLN1-FL-LBD

   Plasmid

   #164839

   Purpose

   Expression of GFP tagged FL mouse talin1 mutant

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

   Insert

   TLN1 (Tln1 Mouse)
   

   Tags

   His8-GFP

   Expression

   Mammalian

   Mutation

   Lipid binding deficient mutations

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T MDM2 S186D

   Plasmid

   #16239

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Ser186 replaced by Asp

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

7. p5B3-T

   Plasmid

   #92021

   Purpose

   Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lysA with N-terminal TEV protease cleavage site
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

   Plasmid

   #182349

   Purpose

   mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T MDM2 S166D

   Plasmid

   #16238

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Ser166 replaced by Asp

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pMK1224

    Plasmid

    #84221

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLBPres

    Plasmid

    #82454

    Purpose

    Encodes synthesized SLBP that is resistant to SMARTpool siRNA

    Depositor

    Bharat Ramratnam

    Article

    Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

    Insert

    SLBP (SLBP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pNP1 OR sfGFP

    Plasmid

    #107364

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    OR gate + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AV4-EF1-GFP

    Plasmid

    #124971

    Purpose

    Control for AV4-EF1-NMNAT2

    Depositor

    Kazuhiro Oka

    Article

    Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

    Insert

    GFP
    

    Use

    AAV

    Promoter

    EF1-alpha

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNL1 Ecarin

    Plasmid

    #106289

    Purpose

    Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Ecarin
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5A1-Ti

    Plasmid

    #92018

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V, C151A

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRKVSV FRNK

    Plasmid

    #50534

    Depositor

    Kenneth Yamada

    Article

    Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

    Insert

    protein tyrosine kinase, domain
    

    Tags

    2X VSV

    Expression

    Mammalian

    Mutation

    truncated (NT2186-3268)

    Promoter

    CMV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNP1 AND gate sfGFP

    Plasmid

    #107361

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    AND gate + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5A3-T

    Plasmid

    #92019

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRKVSV FAT

    Plasmid

    #50533

    Depositor

    Kenneth Yamada

    Article

    Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

    Insert

    protein tyrosine kinase, domain
    

    Tags

    2X VSV

    Expression

    Mammalian

    Mutation

    truncated (NT2630-3268)

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. AIRAP 6: AIRAP(1-89).GST,V1.pcDNA3

    Plasmid

    #21796

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    AIRAP (Zfand2a Mouse)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    AA 1-89

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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