We narrowed to 2,359 results for: Efs

1. pcDNA3.1-Letm1

   Plasmid

   #191962

   Purpose

   Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).

   Depositor

   Luca Scorrano

   Insert

   Letm1 (LETM1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB2076 pAAV REPAIR.t1

   Plasmid

   #176323

   Purpose

   pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt1 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   Cas13bt1 crRNA + BpiI cloning site
   

   Use

   AAV and CRISPR

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   E488Q, only the deaminase domain (aa 276-702 are …

   Promoter

   EFS (short EF1alpha) and hU6

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIN-PAmCherry-mSNCA-NE

   Plasmid

   #102364

   Purpose

   Lentiviral overexpression of PA-mCherry synuclein alpha fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   synuclein alpha (Snca Mouse)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-hSNCA-NE

   Plasmid

   #102366

   Purpose

   Lentiviral overexpression of synuclein alpha

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   A silent mutation (333bp downstream of ATG) was i…

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer-myc-ERK1-R84S

   Plasmid

   #213591

   Purpose

   Doxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R84S

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CBP core-dCas9-CBP core

   Plasmid

   #179560

   Purpose

   encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer-myc-ERK1-S170D

   Plasmid

   #213592

   Purpose

   Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   S170D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_EEF1A2_GTP-EFTU-D2

   Plasmid

   #109919

   Purpose

   Protein expression and purification of EEF1A2_GTP-EFTU-D2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EEF1A2_GTP-EFTU-D2 (EEF1A2, EEF1A1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1_DVK_EF

   Plasmid

   #188947

   Purpose

   Level 1 destination vector containing pBBR1 origin of replication and CIDAR EF scars

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-pHCountdown

    Plasmid

    #182301

    Purpose

    lentiviral plasmid encoding pH-Countdown and puromycin selection

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH countdown
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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