We narrowed to 21,668 results for: Tes

1. mVenus-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173864

   Purpose

   Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mVenus-NES-SspB-MCS-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBstSK+IRES-V5-IPpoI (mutant)

   Plasmid

   #206322

   Purpose

   A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

   Depositor

   Brian McStay

   Article

   van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

   Insert

   IRES element-V5 epitope tag-mutated IPpoI open reading frame
   

   Use

   Mrna production

   Tags

   V5-IPpoI fusion protein (mutant version)

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Nxph4-4Q-HA

   Plasmid

   #128104

   Purpose

   Express HA tagged Nxph4-4Q

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-TPI_PTC160-xrRNA-4H

   Plasmid

   #108369

   Purpose

   Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PTC160

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPLN-hFluc-DM-eGFP-KDEL

   Plasmid

   #177728

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Use

   Tags

   KDEL, Prolactin Signal Sequence, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1 DICER1 long-mut-let-7

   Plasmid

   #21651

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant let-7 microRNA binding sites

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL3

   Plasmid

   #184131

   Purpose

   For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Pges-1 (3.3kb)

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIS1 DICER1 long-mut-all

   Plasmid

   #21653

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutated let-7 and miR103/107 microRNA binding sit…

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIS1 DICER1 long-mut-miR103/107

   Plasmid

   #21652

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant miR103/107 microRNA binding sites

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIS1 IMP-1 long-mut-let-7

    Plasmid

    #21642

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR-B/c

    Plasmid

    #227962

    Purpose

    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMDC32B-B/c

    Plasmid

    #227963

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. A1-LCD -3D+3N

    Plasmid

    #234614

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_3DN (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214N, D242N, D250N

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. A1-LCD -4D+4N

    Plasmid

    #234611

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_4DN (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214N, D242N, D250N, D262N

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. A1-LCD -4D+4V

    Plasmid

    #234610

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_4DV (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214V, D242V, D250V, D262V

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A1-LCD -3D+3V

    Plasmid

    #234613

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_3DV (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214V, D242V, D250V

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

    Plasmid

    #217717

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2453

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

    Plasmid

    #217715

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB2 (BAI2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-917

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

    Plasmid

    #217743

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5896

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

    Plasmid

    #217742

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-412

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

    Plasmid

    #217716

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB3 (BAI3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-862

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HA-MBP-TEVpCS-ADGRG4 CTF-FLAG

    Plasmid

    #217702

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG4 (ADGRG4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2721

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA-MBP-TEVpCS-ADGRD1 CTF-FLAG

    Plasmid

    #217687

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRD1 (ADGRD1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-544

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

    Plasmid

    #217685

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR2 (CELSR2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2356

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-MBP-TEVpCS-ADGRC1 CTF-FLAG

    Plasmid

    #217684

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2447

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-MBP-TEVpCS-ADGRG1 CTFdeltaTA-FLAG

    Plasmid

    #217732

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG1 (ADGRG1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-388

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

    Plasmid

    #217683

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB3 (BAI3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-856

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

    Plasmid

    #217710

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRV1 (ADGRV1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-5890

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge