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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,463 results for: T2A

Showing: 1641 - 1660 of 2463 results

  1. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158413-plasmid-map-sequence-id-337077

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  5. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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  6. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Tags
    His tag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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  12. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    134842-plasmid-map-sequence-id-266572

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  13. pAK_DR30_EF1a_CasRx_Hygro

    Plasmid
    #134841
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    134841-plasmid-map-sequence-id-311015

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  14. pAdx-CMV-iCre-P2A-copGFP

    Plasmid
    #73350
    Purpose
    Express iCre and GFP
    Depositor
    Kazuhiro Oka
    Insert
    CMV-copGFP-T2A-iCre
    Use
    Adenoviral
    Promoter
    CMV
    Available Since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    73350-plasmid-map-sequence-id-244382

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  15. pTol2-Synapse reporters

    Plasmid
    #196899
    Purpose
    zebrafish expression
    Depositor
    Reinhard Köster
    Insert
    Synapse reporters
    Use
    Zebrafish expression
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196899-plasmid-map-sequence-id-386836

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  16. pAAV-EF1a-DIO-HTB

    Plasmid
    #44187
    Depositor
    Edward Callaway
    Insert
    Histone tagged GFP, T2A-TVA-E2A-B19G
    Use
    AAV; Adeno-associated vector
    Tags
    GFP
    Promoter
    EF1a
    Available Since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. DONOR plasmid

    Plasmid
    #236552
    Purpose
    Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag
    Expression
    Mammalian
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236552-plasmid-map-sequence-id-469275

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  18. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  19. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Bernhard Schmierer
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196724-plasmid-map-sequence-id-388065

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  20. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid
    #111152
    Purpose
    Chondrocyte specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Mutation
    wt
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111152-plasmid-map-sequence-id-211539

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Showing: 1641 - 1660 of 2463 results