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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-COM

Plasmid

#131226

Purpose

Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

Depositor

Baisong Lu

Publication

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

COM

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Hygro/shp16 (w192-1)

Plasmid

#22264

Depositor

Eric Campeau

Insert

cyclin-dependent kinase inhibitor 2A

Use

Lentiviral and RNAi

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Publication

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-1 shRev-Erbalpha

Plasmid

#22747

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shRev-Erbalpha (Nr1d1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLV.PARP1#5

Plasmid

#14548

Depositor

Didier Trono

Publication

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p5188 pSUPER-shRNA-Brd4 (CT)

Plasmid

#24746

Depositor

Peter Howley

Publication

Schweiger et al J Virol. 2006 May . 80(9):4276-85.

Insert

shRNA-Brd4 (CT)

Use

Retroviral

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-EGFP/RFP-miR-Scrint

Plasmid

#19821

Depositor

Zuoshang Xu

Publication

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

Scrambled miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
lentiviral shRNA GEF-H1

Plasmid

#21477

Depositor

Richard Huganir

Publication

Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-Csy4-PE2

Plasmid

#172667

Purpose

Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

Depositor

Xiaolong Wang

Publication

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Csy4-PE2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Publication

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Publication

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197027

Purpose

Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Publication

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized enRhCas12f1

Expression

Mammalian

Mutation

L270R

Promoter

CBh, CMV, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Publication

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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U6-petRNA

Plasmid

#181802

Purpose

petRNA cloning plasmid

Depositor

Erik Sontheimer

Publication

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

petRNA-Kan

Use

CRISPR

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Publication

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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shRNA-DNMT1i(3'UTR)

Plasmid

#24951

Depositor

Paul Khavari

Publication

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

shRNA DNMT1i targeting the 3′UTR

Use

RNAi and Retroviral

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Publication

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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