We narrowed to 2,485 results for: pcas9

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHsp70-Cas9

Plasmid

#45945

Purpose

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

3x FLAG

Mutation

Silent change from C to A at bp 834 of insert

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCgCas9_recT

Plasmid

#153337

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC5)

Plasmid

#235073

Purpose

Expresses Tad-CBE(CCC5) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC5)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
Tad-CBE(ACC2)

Plasmid

#235061

Purpose

Expresses Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCG3)

Plasmid

#235074

Purpose

Expresses Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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xABEmax

Plasmid

#119813

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC2)

Plasmid

#235072

Purpose

Expresses Tad-CBE(CCC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCJ1021

Plasmid

#228761

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 gRNAs (Ift88 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACC3)

Plasmid

#235062

Purpose

Expresses Tad-CBE(ACC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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