We narrowed to 8,554 results for: sgRNA

1. pK4-K5

   Plasmid

   #218222

   Purpose

   Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK8-J11

   Plasmid

   #218227

   Purpose

   Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK6-K7

   Plasmid

   #218223

   Purpose

   Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG369

   Plasmid

   #165606

   Purpose

   Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

   Expression

   Bacterial

   Mutation

   Recoding of BsaI site in the AmpR cassette, two B…

   Promoter

   lacUV2 driving ω-dCas9 and UV5 driving sgRNA

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-SpCas9-HF1

   Plasmid

   #126762

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR101

   Plasmid

   #187241

   Purpose

   Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR100

    Plasmid

    #187240

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. B-HeFSpCas9

    Plasmid

    #126766

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. B-eSpCas9

    Plasmid

    #126761

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. B-evoSpCas9

    Plasmid

    #126765

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. B-SpCas9

    Plasmid

    #126760

    Purpose

    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. B-HypaSpCas9

    Plasmid

    #126763

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N692A; M694A; Q695A; H698A; amino acids 1005-1013…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAN-OR-MalT-3NT

    Plasmid

    #62309

    Purpose

    synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Inserts

    A2NT sgRNA
    Malt-3NT sgRNA
    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA2, PPhlF, and pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_852

    Plasmid

    #185527

    Purpose

    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and Mouse U6

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HCP4

    Plasmid

    #166106

    Purpose

    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

    Depositor

    Matthias Heinemann

    Insert

    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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