We narrowed to 13,796 results for: CAN
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Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet_N1
Plasmid#85066PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmScarletExpressionMammalianMutationaa1-29PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
ExpressionMammalianPromoterU6Available SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRluc-LC3G120A
Plasmid#105003PurposeMammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.DepositorInsertLC3B (Map1lc3b Rat)
UseLuciferaseTagsRluc with C124A substitutionExpressionMammalianMutationGlycine 120 mutated to alanine, prevents lipidati…Available SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + terminators reporter plasmid
Plasmid#156456PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.DepositorInsertsNeoR/KanR
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet-i_N1
Plasmid#85068PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:RFP
Plasmid#108455PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertTol2-Amp-y-cryst-mCherry
UseGatewayAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:venusGFP
Plasmid#108462PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertiTol2Amp crystGFP
Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-EGxxFP-iP-A
Plasmid#204624PurposeCloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsPromotermU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-2xESP3I-IRES-mCherry
Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneTagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
FU- dio Teal-gephyrin - W
Plasmid#73918PurposeTo express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.DepositorInsertTeal-gephyrin
UseLentiviralTagsTealExpressionMammalianAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsPromoterhH1Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY2ShHELIX_sgRNA_entry (CJT30)
Plasmid#181781PurposeExpresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertY2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationY2-nAniI = F13Y, S111Y, K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 Luc2-IRES-GFP
Plasmid#224467PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFPDepositorInsertLuciferase
UseLentiviralPromoterEF1aAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only