We narrowed to 30,130 results for: REP

1. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarlet-I-mTurquoise2

   Plasmid

   #98839

   Purpose

   Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mScarletI-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbS8k-RFP_N99D

   Plasmid

   #109410

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   N99D repA mutation to increase copy number

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK462 (Hygro-P2A-mAB)

   Plasmid

   #214388

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Hygro-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. A1-20B1(P004)

   Plasmid

   #184056

   Purpose

   This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

   Depositor

   C. Ron Yu

   Article

   Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

   Insert

   A1-20B1 preamplifier
   

   Use

   Synthetic Biology

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-EGFP-TARG1

   Plasmid

   #172586

   Purpose

   For transient expression of N-terminal GFP-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-ymScarletI-URA3

   Plasmid

   #168055

   Purpose

   Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI
   

   Tags

   ymScarletI

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK445 (BSD-P2A-BromoTag)

   Plasmid

   #214375

   Purpose

   BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPLV_C1

   Plasmid

   #186415

   Purpose

   Endogenous murine RNase inhibitor production for cell-free lysate

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

   Insert

   Murine RNase Inhibitor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-UAS-Dendra2

    Plasmid

    #97145

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Dendra2
    

    Use

    Zebrafish

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_SM-FLuc0-pA

    Plasmid

    #210583

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal FLuc
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PNCA-GFP-dYY1

    Plasmid

    #67791

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

    Insert

    MMLV-LTR-GFP
    

    Mutation

    Deletion of LTR U3 YY1 binding domain

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pMK463 (BSD-P2A-mAB)

    Plasmid

    #214389

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ac5-STABLE2-puro

    Plasmid

    #208032

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DepoPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK465 (mAB-Hygro)

    Plasmid

    #214391

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BrpmpTag-Hygro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK449 (BromoTag-mClover-BSD)

    Plasmid

    #214379

    Purpose

    BromoTag-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mClover-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMK464 (mAB-Neo)

    Plasmid

    #214390

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BromoTag-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Expression

    Mammalian

    Promoter

    Tet ON 3G promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK466 (mAB-BSD)

    Plasmid

    #214392

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BromoTag-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. Flag Rab12 Q101L

    Plasmid

    #86788

    Purpose

    mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

    Depositor

    Peter McPherson

    Article

    Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

    Insert

    Rab12 (RAB12 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q101L, constitutively GTP-bound mutant

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMK484 (2mAID-Hygro)

    Plasmid

    #214396

    Purpose

    2mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    2mAID-Hygro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMK487 (Hygro-P2A-3mAID)

    Plasmid

    #214399

    Purpose

    3mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-3mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMK450 (BromoTag-mCherry-BSD)

    Plasmid

    #214380

    Purpose

    BromoTag-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mCherry-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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