We narrowed to 29,795 results for: REP

1. pCMT-flp

   Plasmid

   #67274

   Purpose

   Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA, flp, repA101, oriR101
   

   Use

   Flp recombinase plasmid, temperature sensitive

   Tags

   His-SUMO (flp)

   Expression

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-FKBP12-C1

   Plasmid

   #67900

   Purpose

   Expresses a fusion of mCherry with FKBP12 in mammalian cells

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   mCherry-FKBP12
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Apex2-Venus-CAAX

   Plasmid

   #168511

   Purpose

   Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

   Insert

   Apex2
   

   Use

   Tags

   Venus-CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PARP9-c024

   Plasmid

   #173096

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G102-K298

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-hEF1a-MG

   Plasmid

   #175504

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Parp14-c010

    Plasmid

    #173100

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    F1208-G1388

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSETA-qmTQ2-ATP

    Plasmid

    #225247

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSSmOrange-mKate2

    Plasmid

    #99868

    Purpose

    Measure FRET between LSSmOrange and mKate2

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    LSSmOrange
    

    Use

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. modRNAc0-p53DD

    Plasmid

    #176902

    Purpose

    IVT template plasmid for making p53DD modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    p53DD
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p.UTA.3.0 Empty

    Plasmid

    #82447

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMT-3P2A

    Plasmid

    #111772

    Purpose

    Quadcistronic construct: Co-express four genes by 3 P2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pH-tdGFP

    Plasmid

    #74322

    Purpose

    pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

    Depositor

    Joerg Stelling

    Article

    Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

    Insert

    pH-tdGFP
    

    Use

    Yeast tagging

    Tags

    none

    Expression

    Yeast

    Mutation

    N149Y, Q204H

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. mNeonGreen-2A-mTurquoise2

    Plasmid

    #98885

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. mScarlet-I-mTurquoise2

    Plasmid

    #98839

    Purpose

    Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mScarletI-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-SWAP70

    Plasmid

    #84581

    Purpose

    Expresses GFP-tagged SWAP70 in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 (SWAP70 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pCDB179-LCC

    Plasmid

    #218686

    Purpose

    Encodes codon optimized LCC enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMK467 (dTAG-BromoTag-mAID-EGFP)

    Plasmid

    #214393

    Purpose

    dTAG-BromoTag-mAID-EGFP reporter (TOL2)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-BromoTag-mAID-EGFP reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. env8 reverse J1/13

    Plasmid

    #99831

    Purpose

    GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

    Depositor

    Robert Batey

    Article

    Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

    Insert

    env8 reverse J1/13
    

    Use

    Tags

    GFPuv

    Expression

    Bacterial

    Mutation

    J1/13 sequence is reversed

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pZY101

    Plasmid

    #73932

    Purpose

    This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

    Depositor

    Zhanyuan Zhang

    Article

    Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

    Insert

    bar
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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