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We narrowed to 6,260 results for: pcDNA 3.1

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Showing: 2491 - 2520 of 6260 results

  5. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid
    #228400
    Purpose
    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    NES/PKI-mNeonGreen2x-NLS/FUS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228400-plasmid-map-sequence-id-457259

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  6. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

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  7. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Expression
    Mammalian
    Promoter
    CMV and NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

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  8. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid
    #50914
    Purpose
    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C N-term deletion of aa13-222 in hNKCC1…
    Promoter
    CMV
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50914-plasmid-map-sequence-id-76387

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  9. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid
    #50865
    Purpose
    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C C723S C724V A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50865-plasmid-map-sequence-id-76338

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  10. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid
    #50913
    Purpose
    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S150N S155Q S170Q T177A S183R S193E T203A T205A T…
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50913-plasmid-map-sequence-id-76386

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  12. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182686-plasmid-map-sequence-id-363076

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  13. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50912-plasmid-map-sequence-id-76385

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  14. pcDNA-HisFlag-rUSP19CSdomains

    Plasmid
    #155240
    Purpose
    for mammalian overexpression of the CS domains of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CS domains) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155240-plasmid-map-sequence-id-311847

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  15. pcDNA-HisFlag-rUSP19CYT

    Plasmid
    #155238
    Purpose
    for mammalian overexpression of the cytoplasmic isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155238-plasmid-map-sequence-id-311857

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  16. pcmCherry 3.1(-)

    Plasmid
    #62803
    Purpose
    Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry
    Depositor
    Michal Minczuk
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    62803-plasmid-map-sequence-id-103515

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  17. pcDNA-HA FoxP3

    Plasmid
    #229615
    Purpose
    Mammalian expression of full length mouse FoxP3
    Depositor
    Sun Hur
    Insert
    full length FoxP3 (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229615-plasmid-map-sequence-id-457337

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  18. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid
    #69058
    Purpose
    Dual Fluorescent Reporter for F-actin and Nuclei
    Depositor
    Olivier Pertz
    Insert
    LifeAct-GFP_IRES_NLS-mCherry
    Tags
    Lifeact-GFP, NLS-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69058-plasmid-map-sequence-id-123543

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  19. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155251-plasmid-map-sequence-id-313953

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  20. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available Since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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  21. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  22. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  23. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  24. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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  28. KP.3.1.1

    Plasmid
    #233342
    Purpose
    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3.1.1) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    S31del on the basis of KP.3
    Promoter
    CMV
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233342-plasmid-map-sequence-id-472416

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Showing: 2491 - 2520 of 6260 results