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Plasmid#176103PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-WT PHGDH
Plasmid#83901Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH
Plasmid#83907Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH-C234S
Plasmid#107724PurposeExpression of PHGDH C234S in bacteriaDepositorInsertPHGDH (PHGDH Human)
UseTagsExpressionBacterialMutationC234SPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DDIT3_WT
Plasmid#82107PurposeGateway Donor vector containing DDIT3 , part of the Target Accelerator Plasmid Collection.DepositorInsertDDIT3 (DDIT3 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1-sgPHGDH-G5
Plasmid#83913Purposestable knockoutDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-C234S PHGDH
Plasmid#83902Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationquikchanged cysteine 234 to serinePromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH V261M
Plasmid#212999Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationV261MPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF13B-Fc(DAPA)-AviTag-6xHis
Plasmid#156561PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF13B (TNFRSF13B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH T78A
Plasmid#212997Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationT78APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH R135W
Plasmid#212998Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationR135WPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH T57A
Plasmid#212996Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationT57APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only