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Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-EB3N-VVDfast-mCl3-iLID
Plasmid#190169PurposeDoxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseLentiviralTags-VVDfast-mClover3-iLIDExpressionMutation1-189 aa from EB3PromoterTREAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR LAT/Zap70 iLID-Drop
Plasmid#171038PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.DepositorInsertLAT (LAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Foxf1 HA pShuttle
Plasmid#122120PurposeExpression of Foxf1 in mammalian cellsDepositorInsertFoxf1 (Foxf1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRluc-LC3G120A
Plasmid#105003PurposeMammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.DepositorInsertLC3B (Map1lc3b Rat)
UseLuciferaseTagsRluc with C124A substitutionExpressionMammalianMutationGlycine 120 mutated to alanine, prevents lipidati…PromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-RMA-IRES-EGFP
Plasmid#189629PurposeExpresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterIRES and hSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS
Plasmid#62861PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-b3
Plasmid#189797PurposeExpression of full length human integrin beta 3DepositorInsertintegrin b3 (ITGB3 Human)
UseTags-P2A-mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_EBFP_Donor
Plasmid#187455PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N EBFP donor (ATP1A1 Human)
UseTagsExpressionMammalianMutationPromoterhPGK1Available sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag1-3HABeta2-mEos2
Plasmid#114187Purposestudying clustering of Beta2 receptors by superresolution microscopy (PALM)DepositorInsertAdrenergic receptor beta 2 (ADRB2 Human)
UseTags3HA, FLAG, and mEOS2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl-sr1(SIRPT1)
Plasmid#75298PurposeExpresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 1-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del2 pET28a
Plasmid#122116PurposeExpression Brg1 amino acids 1-837 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTags6XHis and His-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
UseTagsExpressionBacterial and YeastMutationPromoterTDH3Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z
Plasmid#153543Purposeperform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsmCherryExpressionMammalianMutationNAPromoterCMVAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-delta6xNHL-LIN-41
Plasmid#52722Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 593–868PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only