We narrowed to 4,720 results for: donor

Showing: 1661 - 1680 of 4720 results

Results per page:

Sort results by:

pDONR-ARF19

Plasmid

#83846

Purpose

Gateway donor vector for ARF19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

ARF19 (ARF19 Mustard Weed)

Use

Gateway Cloning

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHD-SPARC2-S-LexA::p65

Plasmid

#133564

Purpose

Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-actin-R62D

Plasmid

#118379

Purpose

Gateway compatible donor vector with actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mNeon-Blast

Plasmid

#207709

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Puro-mScarlet

Plasmid

#207689

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Puro-3xFLAG

Plasmid

#207695

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

Plasmid

#154827

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

TagBFP
HygR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Promoter

CMV, EF1a, and SV40 early promoter

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-SYN_mCyRFP2-CREB

Plasmid

#137008

Purpose

AAV backbone, mCyRFP2-CREB donor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mCyRFP2

Expression

Mammalian

Promoter

hSynapsin

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-SYN_mEGFP-CREB

Plasmid

#137006

Purpose

AAV backbone, mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mammalian

Promoter

hSynapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19/PLIN2-GFP

Plasmid

#202194

Purpose

knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP knock-in

Promoter

lac

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HR-TERT-promotor

Plasmid

#71398

Purpose

Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAav-TP53-T2A-BirA*

Plasmid

#115652

Purpose

rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

Inserts

TP53 (TP53 Human)
TP53 (TP53 Human)
T2A
BirA*

Use

AAV

Tags

BirA* and T2A

Mutation

Homology region 1 and Homology region 2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTF-lacZ-rfp

Plasmid

#153037

Purpose

CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRK246

Plasmid

#173741

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

wrt-2 promoter

Use

Other

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mStayGold-EFS-Blast

Plasmid

#227257

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mNeon-Puro

Plasmid

#207720

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge