We narrowed to 5,818 results for: pCas

1. pCas9-EcRT-GAL-Kan

   Plasmid

   #232092

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   SpCas9 and Ec86 reverse transcriptase
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Ec86 reverse transcriptase is codon optimized for…

   Promoter

   GAL1-GAL10

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-LplA(AILR,36)-NRX3b

   Plasmid

   #43919

   Depositor

   Alice Ting

   Article

   Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

   Insert

   AILR-LplA36-NRX3b (lplA Human, Synthetic)
   

   Tags

   2XHA

   Expression

   Mammalian

   Promoter

   Chicken beta actin

   Available Since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS/XBB.1.16-RBD

   Plasmid

   #213070

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.16-RBD (S )
   

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-pac-T2A-iCre (v2)

   Plasmid

   #125821

   Purpose

   Puromycin resistance gene and iCre recombinase (version 2)

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-iCre
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   ENO1p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

   Plasmid

   #141048

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF1-2A-EGFP
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_GST-TEV-EGFP-ATG13

   Plasmid

   #223797

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Mammalian

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-VSFP Butterfly 1.2

   Plasmid

   #47978

   Purpose

   Genetically encoded voltage indicator

   Depositor

   Thomas Knopfel

   Article

   Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

   Insert

   VSFP Butterfly 1.2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APPs-695-alpha

    Plasmid

    #30147

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein - soluble (APP Human)
    

    Expression

    Mammalian

    Mutation

    amino acids 1–612

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-sfGFP-iCre-ERT2

    Plasmid

    #125749

    Purpose

    sfGFP and iCreERT2 fused by unskippable linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-pac-T2A-FLPo

    Plasmid

    #124835

    Purpose

    Puromycin resistance gene and FLP recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-FLPo
    

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS D614G soluble Spike

    Plasmid

    #164651

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-Tet-on Advanced -BGH-PA

    Plasmid

    #114379

    Purpose

    Express reverse tetracycline inducible rtTA transcriptional activator

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

    Insert

    rtTA and BGH-pA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-pCaMKII-EBFP-tTAn

    Plasmid

    #172124

    Purpose

    Expression of EBFP and tTA-InteinN in CaMKII positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    pCaMKII, EBFP, tTAn-InteinN
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AMPH1-BAR-mCherry

    Plasmid

    #85130

    Purpose

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cmv d0 dPspCas13b-GGS-NYTHDF1

    Plasmid

    #119855

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG mFoxg1-GFP wildtype

    Plasmid

    #73048

    Purpose

    C-terminal fusion of gfp Mouse wildtype Foxg1

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    mFoxg1 (Foxg1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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