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AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

Plasmid

#205443

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46-HLA-G SCT-hCD47-hCD59

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-PH(VPS13C)

Plasmid

#232869

Purpose

Expresses the PH domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the PH domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B WT

Plasmid

#184042

Purpose

Expresses H2B-GFP fused to Aurora B WT

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Tags

EGFP and H2B

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mScarlet-PPM1H

Plasmid

#233585

Purpose

Expresses PPM1H in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

PPM1H (PPM1H Human)

Tags

mScarlet

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9-Gem

Plasmid

#80424

Purpose

Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9-Gem

Use

CRISPR

Tags

Geminin

Mutation

codon optimized for human

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA_NRF2

Plasmid

#136539

Purpose

Lentiviral expression vector for an inducible BirA-NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA-NRF2 (NFE2L2 Human)

Use

Lentiviral; Destination vector for gateway cloning

Expression

Mammalian

Available Since

June 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

Plasmid

#164131

Purpose

Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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wt MPC1-FLAG

Plasmid

#228903

Purpose

Expression of wild-type mouse MPC1 protein with a C terminal FLAG tag

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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